Our Expertise in mRNA Services for Your Discovery Phase

As your mRNA program matures, you will need increasing quality and quantity. RNA has specific challenges, including a rapidly evolving regulatory environment.

We are a proven contract and development manufacturer in the RNA space, providing high-quality mRNA precursors (cloning backbones, linear plasmid DNA templates, in vitro transcription and capping enzymes) and full mRNA synthesis since 2017. Most recently, we’ve expanded our research grade custom manufacturing offering to include:

• Cloning Services for mRNA Backbones
• Linear DNA Template Synthesis using Alchemy™ Cell-free DNA Technology
• Plasmid Preparation and Linearization for IVT
• mRNA synthesis with Codex® HiCap T7 RNA Polymerase
• mRNA-LNP Encapsulation

Our cloning services are designed to support your mRNA research and development needs by providing high-quality cloning backbones tailored for mRNA synthesis. These backbones include essential elements such as UTR sequences, T7 promoters, and Poly(A) tails, ensuring optimal performance in your mRNA projects.

Key Benefits

• Customizable Backbones
  We offer a range of cloning backbones that can be customized to meet the specific requirements of your mRNA synthesis projects.
• High Efficiency
  Our cloning processes are optimized to ensure high efficiency and reliability, providing you with robust and reproducible results.
• Quality Assurance
  Each backbone undergoes rigorous quality control measures to ensure it meets the highest standards of purity and functionality.
• Expert Support
  Our team of experts is available to assist you with the design and implementation of your cloning strategies, ensuring your mRNA projects are successful from start to finish.
• Comprehensive Services
  We deliver end-to-end services to support your mRNA production needs, whether you're working with small-scale research batches or advancing toward commercial-scale manufacturing.

Specialized services for producing linear DNA templates from DNA plasmid or our NEW Alchemy™ Cell-free DNA Technology, which are essential for mRNA production.

Key Benefits

• Rapid Production
  Utilizing advanced technologies like cell-free enzymatic synthesis and rolling circle amplification, we can produce linear DNA templates quickly, often reducing production times significantly.
• High Purity and Quality
  Our production processes are designed to minimize impurities, such as endotoxins and residual proteins, ensuring high-purity DNA suitable for mRNA synthesis.
• Scalability
  We offer scalable solutions, from small-scale research and preclinical batches to large-scale commercial production, supporting various stages of drug development.
• Comprehensive Services
  We provide end-to-end services, including sequence verification, quantitative analysis, structural integrity assessment, and impurity analysis, to support the entire lifecycle of mRNA drug development.

Our specialized services for producing linear DNA templates from plasmid DNA are designed to meet the specific needs of mRNA production.

Key Benefits

• Efficient Production
  Using advanced techniques, we can efficiently convert plasmid DNA into high-quality linear DNA templates.
• High Purity
  Our processes ensure that the linear DNA templates are free from impurities, making them ideal for mRNA synthesis.
• Custom Solutions
  We offer tailored solutions to meet the unique requirements of your mRNA projects, from small-scale research to large-scale commercial production.

Aldevron’s Research Grade RNA manufacturing is now utilizing Codex HiCap as the standard to deliver top quality mRNA with no additional costs.

Codex HiCap T7 RNA Polymerase is a co-transcriptional capping RNA polymerase engineered to produce synthetic RNA to meet increasing demands for safety and cost-effectiveness.

Key Benefits

• Improved Capping Efficiency
  Codex® HiCap T7 RNA Polymerase incorporates commercially available cap analogs more efficiently, reducing overall manufacturing costs.
• Reduced Immunogenicity
  This enzyme generates fewer undesirable double-stranded RNA (dsRNA) byproducts than wild-type T7 RNA polymerase, reducing unwanted immunogenicity.
• High Yield
  Achieve exceptional in vitro transcription (IVT) results with high yield and low immunogenicity.

Aldevron's mRNA-LNP encapsulation capabilities support early process development using Cytiva's NanoAssemblr™ Ignite instrumentation, facilitating the transition to early preclinical stages and beyond.

Key Benefits

• Controlled Assembly
  The NanoAssemblr™ Ignite system enables precise control over fluid flow rates and ratios, allowing fine-tuning of critical quality attributes (CQAs) such as particle size.
• Scalability
  The system is scalable, enabling modeling of clinically relevant parameters and processes at small volumes to predictably scale optimized formulations.
• Reproducibility
  Automated and advanced NxGen™ mixing technology minimizes batch-to-batch and user variability, ensuring reproducible results.
• Ease of Use
  Simple workflows and intuitive software allow users to be up and running quickly, minimizing troubleshooting and downtime.

These research grade solutions enable researchers and pharmaceutical companies to accelerate their mRNA projects with reliable, reliable mRNA backbones, high-quality linear DNA templates, mRNA production, and LNP encapsulation.

Supporting Research Grade RNA Products

• Wild type IVT enzymes for in vitro transcription, capping, and tailing
• DasherGFP® mRNA reporter