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Codex® HiCap RNA Polymerase
in vitro transcription for advancing RNA medicine
Codex® HiCap RNA Polymerase is a co-transcriptional capping RNA polymerase engineered to produce synthetic RNA to meet increasing demands for safety and cost-effectiveness. The HiCap enzyme generates fewer undesirable double-stranded RNA (dsRNA) byproducts than wild-type T7 (WT T7) RNA polymerase, reducing unwanted immunogenicity. In addition, the enzyme incorporates commercially available cap analogs more efficiently, reducing overall manufacturing costs.
dsRNA byproducts
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Replicate IVTs were prepared using a ~2kb plasmid DNA template and either WT T7 or Codex®HiCap RNA Polymerase with Anti-Reverse Cap Analog (ARCA) or CleanCap AG. All IVTs were incubated for 2 hrs at 37°C . Both 2.5 and 25µg of the resulting raw mRNA were evaluated for presence of dsRNA byproducts by dot blot using J2 dsRNA specific primary antibody.
Capping Efficiency
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WT T7 IVTs were prepared with 5mM ARCA, and 1mM GTP or 4mM CleanCap AG. Codex® HiCap RNA IVTs were prepared 4mM ARCA, and 5mM GTP or with 1.5 mM CleanCap AG. All IVTs were incubated for 2 hrs at 37°C .mRNA titers were assessed using the Invitrogen Quant-IT RNA BR Assay Kit. IVT samples were further purified using NEB Monach RNA Cleanup kit and evaluated by gel electrophoresis.
Download our brochure for more performance data.
Codex® HiCap RNA Polymerase vs. Wild-type T7
Codex® HiCap RNA Polymerase | Wild-type T7 polymerase | |
Reduced dsRNA Production | +++ | + |
Co-transcriptional capping efficiency | +++ | ++ |
IVT yields | +++ | +++ |
Manufacturing simplification | +++ | + |
Finished Product Yield | +++ | + |
Codex® HiCap RNA Polymerase Resources
- Codex® HiCap RNA Polymerase Brochure
- Codex® HiCap RNA Polymerase in vitro Transcription Procedure
- Research Article: An Engineered T7 RNA Polymerase for efficient co-transcriptional capping with reduced dsRNA byproducts in mRNA synthesis
- Press Release: Codexis and Aldevron Enter Exclusive Licensing Agreement for Codex® HiCap RNA Polymerase
Ordering Information
Catalog # | Product Description | Size |
E014-B032A-250 µL | Codex® HiCap RNA Polymerase | 250 µl |
E014-B032A-1mL | Codex® HiCap RNA Polymerase | 1 ml |
E014-B032A-10mL | Codex® HiCap RNA Polymerase | 10 ml |
*Codex® is a trademark of Codexis, Inc. Any other third-party trademarks are the property of their respective owners.