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Wild-type IVT & Capping Enzymes

Enzymes for mRNA Production

Don’t trust your large-scale in vitro transcription (IVT) reactions to unproven enzymes. At Aldevron, we are our own biggest client for IVT enzymes. Using Aldevron-manufactured IVT enzymes, we have delivered high-quality mRNA for our clients since 2017, manufacturing hundreds of research-grade batches and >50 GMP batches, in support of numerous IND submissions and late phase clinical programs.

Our research-grade catalog enzymes are manufactured in our ISO13485 certified Innovate Campus

Custom in vitro transcription enzymes can be manufactured at research-grade though our custom manufacturing service.

Transcription
Product Name Product Description
T7 RNA Polymerase T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter.
Inorganic Pyrophosphatase Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate.
Ribonuclease Inhibitor Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C.
DNase I DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.
Capping
Product Name Product Description
Guanylyltransferase Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:

Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.

Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
2’-O-Methyltransferase 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes
S-adenosylmethionine as the methyl group donor.
Tailing
Product Name Product Description
Poly(A) Polymerase Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner.

Resources

Ordering Information

Product Pack Size Catalog Number RG Purchase Option
2’-O-Methyltransferase - RG 1450 µg 9130-1450MCG Buy Now
2’-O-Methyltransferase - RG 3400 µg 9130-3400MCG Buy Now
Guanylyltransferase - RG 300 µg 9131-300MCG Buy Now
Guanylyltransferase - RG 1750 µg 9131-1750MCG Buy Now
Inorganic Pyrophosphatase - RG 350 µg 9132-350MCG Buy Now
Inorganic Pyrophosphatase - RG 1870 µg 9132-1870MCG Buy Now
Poly(A) Polymerase - RG 430 µg 9133-430MCG Buy Now
Poly(A) Polymerase - RG 3000 µg 9133-3000MCG Buy Now
Ribonuclease Inhibitor - RG 660 µg 9134-660MCG Buy Now
Ribonuclease Inhibitor - RG 2500 µg 9134-2500MCG Buy Now
T7 RNA Polymerase - RG 130 µg 9135-130MCG Buy Now
T7 RNA Polymerase - RG 2000 µg 9135-2000MCG Buy Now
DNase I - RG 70 µg 9136-70MCG Buy Now
DNase I - RG 500 µg 9136-500MCG Buy Now

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