Wild-type IVT & Capping Enzymes
Enzymes for mRNA Production
Don’t trust your large-scale in vitro transcription (IVT) reactions to unproven enzymes. At Aldevron, we are our own biggest client for IVT enzymes. Using Aldevron-manufactured IVT enzymes, we have delivered high-quality mRNA for our clients since 2017, manufacturing hundreds of research-grade batches and >50 GMP batches, in support of numerous IND submissions and late phase clinical programs.
Our catalog enzymes are:
- Research-grade material is manufactured in our ISO13485 certified Innovate Campus
- Anticipated to be available at GMP grade as demand warrants
Custom in vitro transcription enzymes can be manufactured at research or GMP grade though our custom manufacturing service.
Transcription
| Product Name | Product Description |
| T7 RNA Polymerase | T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter. |
| Inorganic Pyrophosphatase | Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate. |
| Ribonuclease Inhibitor | Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C. |
| DNase I | DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity. |
Capping
| Product Name | Product Description |
| Guanylyltransferase | Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps: Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C. Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate. |
| 2’-O-Methyltransferase | 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor. |
Tailing
| Product Name | Product Description |
| Poly(A) Polymerase | Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner. |
Resources and Ordering Information
Resources
- Download our IVT Technical Guide (PDF)
- See our RNA Services
- Contact our RNA team for Custom mRNA or Linearized pDNA Manufacturing
Ordering Information
| IVT Enzymes | Catalog Number | Small Size | Large Sizes |
| 2’-O-Methyltransferase | 9130 | 1450 mcg | 3400 mcg |
| Guanylyltransferase | 9131 | 300 mcg | 1750 mcg |
| Inorganic Pyrophosphatase | 9132 | 350 mcg | 1870 mcg |
| Poly(A) Polymerase | 9133 | 430 mcg | 3000 mcg |
| Ribonuclease Inhibitor | 9134 | 660 mcg | 2500 mcg |
| T7 RNA Polymerase | 9135 | 130 mcg | 2000 mcg |
| DNase I | 9136 | 70 mcg | 500 mcg |
Interested in a sample, pricing, placing an order, or custom manufacturing?