Wild-type IVT & Capping Enzymes
Enzymes for mRNA Production
Don’t trust your large-scale in vitro transcription (IVT) reactions to unproven enzymes. At Aldevron, we are our own biggest client for IVT enzymes. Using Aldevron-manufactured IVT enzymes, we have delivered high-quality mRNA for our clients since 2017, manufacturing hundreds of research grade batches and >50 GMP batches, in support of numerous IND submissions and late phase clinical programs.
Our research grade catalog enzymes are manufactured in our ISO13485 certified Innovate Campus
Custom in vitro transcription enzymes can be manufactured at research-grade though our custom manufacturing service.
Transcription
| Product Name | Product Description |
| T7 RNA Polymerase | T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter. |
| Inorganic Pyrophosphatase | Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate. |
| Ribonuclease Inhibitor | Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C. |
| DNase I | DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity. |
Capping
| Product Name | Product Description |
| Guanylyltransferase | Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps: Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C. Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate. |
| 2’-O-Methyltransferase | 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor. |
Tailing
| Product Name | Product Description |
| Poly(A) Polymerase | Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner. |
Resources
- Download our IVT Technical Guide (PDF)
- See our RNA Services
- Contact our RNA team for Custom mRNA or Linearized pDNA Manufacturing
Ordering Information
| Product | Pack Size | Catalog Number | RG Purchase Option |
| 2’-O-Methyltransferase - RG | 1450 µg | 9130-1450MCG | Buy Now |
| 2’-O-Methyltransferase - RG | 3400 µg | 9130-3400MCG | Buy Now |
| Guanylyltransferase - RG | 300 µg | 9131-300MCG | Buy Now |
| Guanylyltransferase - RG | 1750 µg | 9131-1750MCG | Buy Now |
| Inorganic Pyrophosphatase - RG | 350 µg | 9132-350MCG | Buy Now |
| Inorganic Pyrophosphatase - RG | 1870 µg | 9132-1870MCG | Buy Now |
| Poly(A) Polymerase - RG | 430 µg | 9133-430MCG | Buy Now |
| Poly(A) Polymerase - RG | 3000 µg | 9133-3000MCG | Buy Now |
| Ribonuclease Inhibitor - RG | 660 µg | 9134-660MCG | Buy Now |
| Ribonuclease Inhibitor - RG | 2500 µg | 9134-2500MCG | Buy Now |
| T7 RNA Polymerase - RG | 130 µg | 9135-130MCG | Buy Now |
| T7 RNA Polymerase - RG | 2000 µg | 9135-2000MCG | Buy Now |
| DNase I - RG | 70 µg | 9136-70MCG | Buy Now |
| DNase I - RG | 500 µg | 9136-500MCG | Buy Now |
Not ready to place an order? Request a free sample to try it in your workflow.
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