
Innovative mRNA Production Techniques for Efficient RNA Drug Development
Enhancing mRNA production begins with stability at the design stage
Imagine having access to a comprehensive range of specialized products and services for mRNA production that can significantly reduce supply-chain risks, shorten lead times, and enhance quality. Whether you need simple starting material such as cloning backbones, enzymes for mRNA synthesis in various configurations from research grade to cGMP drug substance and product, or specific options for capping, tailing, and chemical modification, you can find everything you need to support your program development. mRNA production can begin with an electronic DNA template sequence or supercoiled plasmid DNA provided by the client, supported by process development, scale-up, and analytics required to move your project into clinical trials and beyond.
MRNA construct design and optimization
Aldevron's state-of-the-art quality systems ensure your material meets your specifications. From cloning, verification, restriction digestion, strain selection, cell banking, and plasmid preparation, we meticulously manage every step to guarantee the integrity and efficacy of the mRNA sequence. Whether for research or clinical applications, our commitment to quality ensures the mRNA you receive is of the utmost reliability.
RNA Backbones for Vaccine and Therapeutic Development
- Optimized for streamlining the development of vaccines and therapeutics
- Constructs feature a T7 promoter for RNA production
- Proprietary 5’ UTR sequence to help determine which configuration works best
- Options include encoded poly(A) region
Strain Selection and Optimization
- Test 2-3 E.coli strains
- Generate a design space for process conditions, including multiple temp and media components
- Develop a small-scale model for full-scale production
cGMP Master Cell Bank Generation
- Generate cell bank for use in research and cGMP projects
- Enable faster project execution during late-stage clinical phases
LNP formulation
LNP formulation is important for long-term success of any RNA drug product. We partner with Cytiva and Acuitas to work with you on LNP formulation. These partnerships and capabilities assure that you have access to all the essentials for mRNA vaccine and therapeutic development, including:
- Encapsulation technology and expertise
- A lipid library as an initial step for screening
- Multiple platforms to optimize consistency and encapsulation efficiency
- Scale based to support various stages of development The Aldevron Advantage
Providing our clients with options, from the simplest starting material to the most complex manufacturing operation, is part of our culture. Our aim is to bring innovation to each step of the journey.

Cloning backbones
Clone your gene of interest into one of several Aldevron backbones, some with optimized UTRs, for efficient mRNA production.

Alchemy™ Cell-free DNA
Reduce linear DNA manufacturing times by over 50% and reduce host cell contamination with the Alchemy Cell-free DNA enzymatic manufacturing process

Plasmid DNA and linearization
Produce phase-appropriate plasmid DNA for research through clinical applications at a variety of scales. Recover 90-95% linear DNA with consistently high poly(A) tails through optimized linearization and purification protocols.

Process development and scale up
Utilize experience gained from over 130 cGMP RNA lots produced for clinical trials and commercial applications to optimize process and scale for each unique molecule.

RNA drug substance manufacturing
Flexible, scalable process produces high-quality RNA tailored to your specifications, delivering quantities from milligrams to multi-grams.

RNA LNP drug product
Seamless continuation of the RNA drug product manufacturing process, incorporating LNP encapsulation, analytical testing and sterile fill/finish under a single roof and quality system.
Explore the foundational research and innovative techniques that drive RNA discovery. Learn how Aldevron's cutting-edge science and expertise in target identification and molecule design pave the way for groundbreaking RNA therapeutics.
Resources
- mRNA Services (PDF)
- Alchemy™ Cell-free DNA Technology (PDF)
- Revolutionizing mRNA Manufacturing with Alchemy™ Cell-free DNA Technology (Webinar)
- Mastering the Path: Developing Processes for Successful Clinical-Grade mRNA Manufacturing (Webinar)
- Comprehensive GMP Manufacturing of mRNA-LNP Drug Products: Sequence-to-Vial (Webinar)
- How a Sponsored CMC Platform Accelerates mRNA Projects to the Clinic (Webinar)
- mRNA/saRNA Manufacturing: Considerations to Accelerate the Path to Clinic (Webinar)
- Q&A series with Aldevron CSO, Venkata Indurthi, Ph.D. (PDF)
- Topic 1: Program Development & Support
- Topic 2: Synthesis Process & Methods
- Topic 3: Production & Manufacturing
- Topic 4: Analytics & Testing
- Topic 5: Therapeutic Delivery & Applications