Gene Editing Enzymes

Gene Editing Enzymes

Nucleases for Research and Clinical Applications

There are many commercial nuclease suppliers for basic research and academic purposes. But your goals and needs are different. You aspire to utilize CRISPR gene editing for research or therapeutic purposes and need a product that is going to be available under the appropriate quality systems (research grade, ISO 13485, or full cGMP) as you progress.

Aldevron manufactures and stocks nucleases that can eliminate the need for custom manufacturing runs, as your program moves towards commercialization.

Product Description Drug Master File on File
Eureca-V™ Nuclease* Wild-type MAD7™ ® Type-V nuclease with pass-through licensing* No
SpyFi™ Cas9 Nuclease High fidelity nuclease with less off-target activity Yes
SpCas9 Nuclease Wild-type SpCas9 Yes
AsCas12a Nuclease Wild-type AsCas12a No
SaCas9 Nuclease Wild-type SaCas9 No

*Licensed from Inscripta®

Order Research Grade (RG)

Product Pack Size Catalog Number RG
Purchase Option
Eureca-V™ Nuclease* - RG 0.25 mg 9300-0.25MG Buy Now
Eureca-V™ Nuclease* - RG 1 mg 9300-1MG Buy Now
Eureca-V™ Nuclease* - RG 5 mg 9300-5MG Buy Now
SpyFi™ Cas9 Nuclease - RG 0.25 mg 9214-0.25MG Buy Now
SpyFi™ Cas9 Nuclease - RG 5 mg 9214-5MG Buy Now
SpCas9 Nuclease - RG 0.25 mg 9212-0.25MG Buy Now
SpCas9 Nuclease - RG 5 mg 9212-5MG Buy Now

*Licensed from Inscripta®

Request cGMP or Custom Manufacturing Information

Product Pack Size Catalog Number Purchase Option
SpyFi™ Cas9 Nuclease - cGMP 1 mg 9216-0.1ML Inquire
SpyFi™ Cas9 Nuclease - cGMP 10 mg 9216-1ML Inquire
SpCas9 Nuclease - cGMP 1 mg 9211-0.1ML Inquire
SpCas9 Nuclease - cGMP 10 mg 9211-1ML Inquire
Eureca-V™ Nuclease* - cGMP Custom Inquire
AsCas12a Nuclease - RG Custom Inquire
SaCas9 Nuclease -RG Custom Inquire

*Licensed from Inscripta®

Don’t See What You Need?

Aldevron offers custom manufacture of unique Cas enzyme configurations including dCas9 fusions, nickases, Cas9 variants and non-Cas9 nucleases. For more information visit our Custom Protein Manufacturing page or contact our Custom Nuclease Services group.

Ribonucleoprotein Production**

Aldevron offers an RNP service to streamline transforming your CRISPR reagents into therapies. Working with your unique guide RNAs, we’ve defined the optimal conditions for complexing, characterizing, and storing CRISPR RNPs created with Aldevron research and cGMP-grade CRISPR/Cas9 proteins. Our proprietary release panels are compliant with 21CFR210-211 and designed to meet current draft guidance from the FDA regarding gene editing, which provides consistency of the final product.

Learn more about our RNP Production Services

**Aldevron provides RNPs only to customers who are duly licensed, including to make and have made RNPs, for their intended use.
Resources
Scientific Publications

Publications featuring other Aldevron Nucleases

  • High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA

This publication showed improved CAR-T knock-in efficiency, reduced cell death, and an improved T cell phenotype for CAR-T cell therapies using Nanoplasmid compared to conventional plasmids and linear DNA as an HDR template. Experiments are conducted with SpyFi Cas9 Nuclease.

Oh SA, Senger K, Madireddi S, Akhmetzyanova I, Ishizuka IE, Tarighat S, et al. High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA. Journal of Experimental Medicine. 2022;219(5). https://doi.org/10.1084/jem.20211530

  • Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells

This paper showed GMP-compliant method to manufacture CRISPR gene-edited VSTs that are unaffected by glucocorticoid treatment. These CRISPR-edited VSTs have comparable potency to non-gene edited VSTs but could be co-administered to patients with steroids. The GMP manufacturing workflow applies Aldevron SpyFi™ Cas9 Nuclease as the gene editing reagent due to its high on-target editing efficiency and greatly reduced off-target editing in comparison to wild-type SpCas9.

Basar, R., Daher, M., Uprety, N., Gokdemir, E., Alsuliman, A., Ensley, E., Ozcan, G., Mendt, M., Hernandez Sanabria, M., Kerbauy, L. N., Nunez Cortes, A. K., Li, L., Banerjee, P. P., Muniz-Feliciano, L., Acharya, S., Fowlkes, N. W., Lu, J., Li, S., Mielke, S., Kaplan, M., … Rezvani, K. (2020). Large-scale GMP-compliant CRISPR-Cas9-mediated deletion of the glucocorticoid receptor in multivirus-specific T cells. Blood advances, 4(14), 3357–3367. https://doi.org/10.1182/bloodadvances.2020001977

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