Spy FiTM Cas9 Nuclease

Reduced off-target activity without loss of editing efficiency

From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research Grade, GMP-Source™ and GMP levels.

ald-SpyFi single chart-1217

SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.

Functional Performance

Manufacturing and Quality Control


Aldevron’s SpyFi Cas9 Nuclease is sold under license of patents and/or patents pending from Integrated DNA Technologies, Inc. (IDT). Data shown above was generated in collaboration with IDT.

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SpyFi Quality Control Specifications
Purity, RP-HPLC ≥95%
Purity, SDS-PAGE ≥95%
Aggregation ≤5% aggregate
Concentration 10.0 mg/mL +/- 10%
Endotoxin ≤ 10 EU/mg
Residual DNase < LOQ
Residual RNase < LOQ
Appearance Clear, colorless
Residual Host Protein ≤ 200 ng/mL
Residual Host GenomicDNA < 1% (w/w)
Activity Assay, in vitro ≥80% of uncut reference DNA converted to cleavage products
Bioburden < 2 CFU/Sample
Mycoplasma Negative for the presence of Mycoplasma


Ordering Information

Product Small Size Catalog Number Large Sizes Catalog Number
SpyFi™ Cas9 Nuclease 0.25MG 9214-0.25MG 5MG 9214-5MG
GMP-Manufactured Nucleases
SpyFi™ Cas9 Nuclease – GMP 1MG 9216-0.1ML 10MG 9216-1ML

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