Spy FiTM Cas9 Nuclease
Reduced off-target activity without loss of editing efficiency
From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research Grade, GMP-Source™ and GMP levels.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
- Reduced off-target effects
- Ready to use for electroporation, transfections or injection methods
- 10 mg/mL formulation for ease of use
Manufacturing and Quality Control
- Research grade to GMP
- Consistent activity and purity across all lots
Aldevron’s SpyFi Cas9 Nuclease is sold under license of patents and/or patents pending from Integrated DNA Technologies, Inc. (IDT). Data shown above was generated in collaboration with IDT.
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|10.0 mg/mL +/- 10%
|≤ 10 EU/mg
|Residual Host Protein
|≤ 200 ng/mL
|Residual Host GenomicDNA
|< 1% (w/w)
|Activity Assay, in vitro
|≥80% of uncut reference DNA converted to cleavage products
|< 2 CFU/Sample
|Negative for the presence of Mycoplasma
|SpyFi™ Cas9 Nuclease
|SpyFi™ Cas9 Nuclease – GMP
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