sNLS-SpCas9-sNLS Nuclease
A simple solution from R&D to clinical material
Aldevron provides Research-grade Cas9 nucleases for use in development work as well as standard cGMP products for clinical studies. The concentration for these products is 10 mg/mL, dispensed in the following sizes:
- Research-grade: 0.25 mg and 5 mg vials
- cGMP: 1 mg and 10 mg vials
Functional Performance
- Ready to use for electroporation or transfection
- Extensive QC panels aligned at Research-Grade and cGMP product levels
- 10 mg/mL formulation
Manufacturing and Quality Control
- Research-grade to cGMP
- Consistent activity and purity across lots
Download CoAs to See QC Specifications
To download your CoAs, please provide your contact information below. We do not share your information with any third parties.
sNLS-SpCas9-sNLS Nuclease Quality Control Specifications
| Purity, RP-HPLC | ≥95% |
| Purity, SDS-PAGE | ≥95% |
| Aggregation | ≤5% aggregate |
| Concentration | 10.0 mg/mL +/- 10% |
| Endotoxin | ≤ 10 EU/mg |
| Residual DNase | < LOQ |
| Residual RNase | < LOQ |
| Appearance | Clear, colorless |
| Residual Host Protein | ≤ 200 ng/mL |
| Residual Host GenomicDNA | < 1% (w/w) |
| Activity Assay, in vitro | ≥80% of uncut reference DNA converted to cleavage products |
| Bioburden | < 2 CFU/Sample |
| Mycoplasma | Negative for the presence of Mycoplasma |
Ordering Information
| Product | Small Size | Catalog Number | Large Sizes | Catalog Number |
| SpCas9 Nuclease | 0.25MG | 9212-0.25MG | 5MG | 9212-5MG |
| cGMP-Manufactured Nucleases | ||||
| SpCas9 Nuclease - GMP | 1MG | 9211-0.1ML | 10MG | 9211-1ML |
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WHITEPAPER: Next-generation CRISPR Approaches
CASE STUDY: Enabling Novel CRISPR-Cas9 Delivery
