Gene Editing Enzymes
Nucleases for Research and Clinical Applications
There are many commercial nuclease suppliers for basic research and academic purposes. But your goals and needs are different. You aspire to utilize CRISPR gene editing for research or therapeutic purposes and need a product that is going to be available under the appropriate quality systems (research grade, ISO 13485, or full cGMP) as you progress.
High-efficiency, low-toxicity Cas9 mRNA formats are now available in both wild type and 𝘚𝘱𝘺Fi™ high-fidelity variants for CRISPR genome editing applications.
Aldevron manufactures and stocks nucleases that can eliminate the need for custom manufacturing runs, as your program moves towards commercialization. Select nucleases are also available as mRNA products.
| Product | Description | Drug Master File on File |
| Eureca-V™ Nuclease* | Wild-type MAD7™ ® Type-V nuclease with pass-through licensing* | No |
| 𝘚𝘱𝘺Fi Cas9 Nuclease | High fidelity nuclease with less off-target activity | Yes |
| SpCas9 Nuclease | Wild-type SpCas9 | Yes |
| AsCas12a Nuclease | Wild-type AsCas12a | No |
| SaCas9 Nuclease | Wild-type SaCas9 | No |
*Licensed from Inscripta®
Order Research Grade (RG)
| Product | Pack Size | Catalog Number | RG Purchase Option |
| Eureca-V Nuclease* - RG | 0.25 mg | 9300-0.25MG | Buy Now |
| Eureca-V Nuclease* - RG | 1 mg | 9300-1MG | Buy Now |
| Eureca-V Nuclease* - RG | 5 mg | 9300-5MG | Buy Now |
| 𝘚𝘱𝘺Fi Cas9 Nuclease - RG | 0.25 mg | 9214-0.25MG | Buy Now |
| 𝘚𝘱𝘺Fi Cas9 Nuclease - RG | 5 mg | 9214-5MG | Buy Now |
| SpCas9 Nuclease - RG | 0.25 mg | 9212-0.25MG | Buy Now |
| SpCas9 Nuclease - RG | 5 mg | 9212-5MG | Buy Now |
| SpCas9 mRNA -RG | 20 µg | 3850-0020 | Buy Now |
| SpCas9 mRNA -RG | 100 µg | 3850-0100 | Buy Now |
| SpCas9 mRNA -RG | 1 mg | 3850-1 | Buy Now |
| 𝘚𝘱𝘺Fi Cas9 mRNA - RG | 20 µg | 3851-0020 | Buy Now |
| 𝘚𝘱𝘺Fi Cas9 mRNA - RG | 100 µg | 3851-0100 | Buy Now |
| 𝘚𝘱𝘺Fi Cas9 mRNA - RG | 1 mg | 3851-1 | Buy Now |
*Licensed from Inscripta®
Request cGMP or Custom Manufacturing Information
| Product | Pack Size | Catalog Number | Purchase Option |
| 𝘚𝘱𝘺Fi Cas9 Nuclease - cGMP | 1 mg | 9216-0.1ML | Inquire |
| 𝘚𝘱𝘺Fi Cas9 Nuclease - cGMP | 10 mg | 9216-1ML | Inquire |
| SpCas9 Nuclease - cGMP | 1 mg | 9211-0.1ML | Inquire |
| SpCas9 Nuclease - cGMP | 10 mg | 9211-1ML | Inquire |
| Eureca-V Nuclease* - cGMP | Custom | – | Inquire |
| 𝘚𝘱𝘺Fi Cas9 mRNA Nuclease - cGMP | Custom | – | Inquire |
| SpCas9 mRNA Nuclease - cGMP | Custom | – | Inquire |
| AsCas12a Nuclease - RG | Custom | – | Inquire |
| SaCas9 Nuclease -RG | Custom | – | Inquire |
*Licensed from Inscripta®
Don’t See What You Need?
Aldevron offers custom manufacture of unique Cas enzyme configurations including dCas9 fusions, nickases, Cas9 variants and non-Cas9 nucleases. For more information visit our Custom Protein Manufacturing page or contact our Custom Nuclease Services group.
Ribonucleoprotein Production**
Aldevron offers an RNP service to streamline transforming your CRISPR reagents into therapies. Working with your unique guide RNAs, we’ve defined the optimal conditions for complexing, characterizing, and storing CRISPR RNPs created with Aldevron research and cGMP grade CRISPR/Cas9 proteins. Our proprietary release panels are developed, manufactured, or validated within a quality system designed to support compliance with 21 CFR Parts 210 and 211.
Learn more about our RNP Production Services
- BROCHURE: Advancing CRISPR-based Therapeutic Development
- WHITEPAPER: Next-Generation CRISPR Approaches: Explore Strategies to Advance Therapeutic Programs Supporting Current Regulatory Guidance
- WHITEPAPER: Expectations on the Pathway to GMP for Gene-Modified Cell Therapies
- CASE STUDY: Enabling Novel CRISPR-Cas9 Delivery: A Case Study Supporting Innovative Genomics Insititute
- WEBINAR: Genome Editing Tools: Beyond Discovery
- WEBINAR: CRISPR Cures 2033: Expanding the Public Health Impact of Gene Editing
- ARTICLE: Expediting IND applications with drug master files
- PODCAST: Insights into the Editing of the Human Genome
Publications featuring other Aldevron Nucleases
- High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA
This publication showed improved CAR-T knock-in efficiency, reduced cell death, and an improved T cell phenotype for CAR-T cell therapies using Nanoplasmid compared to conventional plasmids and linear DNA as an HDR template. Experiments are conducted with 𝘚𝘱𝘺Fi Cas9 Nuclease.
Oh SA, Senger K, Madireddi S, Akhmetzyanova I, Ishizuka IE, Tarighat S, et al. High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA. Journal of Experimental Medicine. 2022;219(5). https://doi.org/10.1084/jem.20211530
- Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells
This paper showed GMP-compliant method to manufacture CRISPR gene-edited VSTs that are unaffected by glucocorticoid treatment. These CRISPR-edited VSTs have comparable potency to non-gene edited VSTs but could be co-administered to patients with steroids. The GMP manufacturing workflow applies Aldevron 𝘚𝘱𝘺Fi™ Cas9 Nuclease as the gene editing reagent due to its high on-target editing efficiency and greatly reduced off-target editing in comparison to wild-type SpCas9.
Basar, R., Daher, M., Uprety, N., Gokdemir, E., Alsuliman, A., Ensley, E., Ozcan, G., Mendt, M., Hernandez Sanabria, M., Kerbauy, L. N., Nunez Cortes, A. K., Li, L., Banerjee, P. P., Muniz-Feliciano, L., Acharya, S., Fowlkes, N. W., Lu, J., Li, S., Mielke, S., Kaplan, M., … Rezvani, K. (2020). Large-scale GMP-compliant CRISPR-Cas9-mediated deletion of the glucocorticoid receptor in multivirus-specific T cells. Blood advances, 4(14), 3357–3367. https://doi.org/10.1182/bloodadvances.2020001977
Discuss your project with an expert
Case Study: Enabling Novel CRISPR-Cas9 Delivery
Whitepaper: Next-generation CRISPR Approaches
