IVT Enzymes

IVT Enzymes

Enzymes for mRNA Production

Don’t trust your large-scale in vitro transcription (IVT) reactions to unproven enzymes. At Aldevron, we are our own biggest client for IVT enzymes. Using Aldevron-manufactured IVT enzymes, we have delivered high-quality mRNA for our clients since 2017, manufacturing hundreds of research-grade batches and >50 GMP batches, in support of numerous IND submissions and late phase clinical programs.

Our catalog enzymes are:

Custom in vitro transcription enzymes can be manufactured at research or GMP grade though our custom manufacturing service.

Transcription
Product Name Product Description
T7 RNA Polymerase T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter.
Inorganic Pyrophosphatase Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate.
Ribonuclease Inhibitor Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C.
DNase I DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.
Capping
Product Name Product Description
Guanylyltransferase Guanyltransferase (GT), also known as Vaccinia Capping Enzyme (VCE), catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:

Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.

Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.
2’-O-Methyltransferase 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes
S-adenosylmethionine as the methyl group donor.
Tailing
Product Name Product Description
Poly(A) Polymerase Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner.

Resources and Ordering Information

Resources

Ordering Information

IVT Enzymes Catalog Number Small Size Large Sizes
2’-O-Methyltransferase 9130 1450 mcg 3400 mcg
Guanylyltransferase 9131 300 mcg 1750 mcg
Inorganic Pyrophosphatase 9132 350 mcg 1870 mcg
Poly(A) Polymerase 9133 430 mcg 3000 mcg
Ribonuclease Inhibitor 9134 660 mcg 2500 mcg
T7 RNA Polymerase 9135 130 mcg 2000 mcg
DNase I 9136 70 mcg 500 mcg

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