NanoplasmidTM

NanoplasmidTM Vector System for Genetic Delivery Optimization:

The Power of a Small Backbone for Gene & Cell Therapies

Looking to enhance performance and manufacturing from your DNA backbone, without the regulatory concerns? The small Nanoplasmid backbone fulfills all the requirements. The Nanoplasmid Vector System (<500 bp backbone) provides advantages over existing legacy plasmid DNA backbones and minicircles:

  • High Transgene Expression
  • Increase Manufacturing Yields
  • Improve Safety Profile by Eliminating Antibiotic Resistant Gene
  • Save Time and Cost-Effective

Combining characteristics of reduced size and the RNA-OUT non-antibiotic resistance selection marker, the platform is ideally suited for a range of therapeutic applications. Aldevron can provide customized Nanoplasmid DNA for each client.

Nanoplasmid System Mfg Advantages_fnl

Nanoplasmid Benefits by Applications

 

Benefits

Application

Performance

Enhance Manufacturing: (P)lasmid
or
(E)nd-Product Production

Regulatory Compliant

Increase Transgene Expression

Lower Transfection Toxicity

AAV virus1

X

X

P

X

Lentivirus

X

 

P/E

X

Non-Viral Gene Therapy2

 

X

E

X

DNA Vaccines3

X

 

E

X

HDR/CRISPR Gene Editing4

X

 

E/P

X

Transposons5

 

X

E/P

X

mRNA Vector Production

 

 

P

N/A

Antibiotic-free selection provided by RNA-OUT

The RNA-OUT platform works by repressing the expression of a counter-selectable marker (SacB) from the host chromosome. SacB encodes for the levansucrase enzyme, which creates a toxic environment in the presence of sucrose, which leads to cell death. Transforming the Antibiotic-free Nanoplasmid in the host expresses a 150bp RNA-OUT antisense RNA (ROUT) that binds the SacB mRNA and represses levansucrase expression, prevents toxicity and to maintain cell survival. The RNA-OUT selectable marker can be used to retrofit existing antibiotic resistance DNA plasmids into antibiotic-free vectors counter-selection system.

ALD Nanoplasmid Bacterial Host Genome-1

Connect with our Specialist

Contact us to schedule a consultation with an expert about your Nanoplasmid product development. In the discussion, our team will evaluate the ability to retrofit your vector into the backbone or design the plasmid within the Nanoplasmid construct for your program.

CONTACT a Specialist