Nanoplasmid is a disruptive technology in the clinical and pre-clinical arena. There have been dozens of publications showing off how this small backbone delivers powerful benefits.
- Boye C, Arpag S, Burcus N, Lundberg C, DeClemente S, Heller R, Francis M, Bulysheva A. Cardioporation enhances myocardial gene expression in rat heart. Bioelectrochemistry. 2021 Dec;142:107892. doi: 10.1016/j.bioelechem.2021.107892. Epub 2021 Jul 27. PMID: 34371349.
Showing improved electroporation transfection methods in vivo and in vitro using Nanoplasmid versus conventional plasmids for naked DNA expression.
- Oh SA, Senger K, Madireddi S, Akhmetzyanova I, Ishizuka IE, Tarighat S, et al. High-efficiency nonviral CRISPR/cas9-mediated gene editing of human T cells using plasmid donor DNA. Journal of Experimental Medicine. 2022;219(5). https://doi.org/10.1084/jem.20211530
This publication showed improved CAR-T knock-in efficiency, reduced cell death, and an improved T cell phenotype for CAR-T cell therapies using Nanoplasmid compared to conventional plasmids and linear DNA as an HDR template.
- Bozza M, De Roia A, Correia MP, Berger A, Tuch A, Schmidt A, et al. A nonviral, nonintegrating DNA nanovector platform for the safe, rapid, and persistent manufacture of recombinant T cells. Science Advances. 2021;7(16). doi: 10.1126/sciadv.abf1333
This article discussed how Nanoplasmid was integrated into a rapid and novel manufacturing workflow for the production of CAR-T cell therapies for patients.
For more information on these and other applications, download the complete bibliography.