pALD Cloning Backbones

Find the Right Backbone for your Application

Aldevron, your trusted partner since 1998, has several cloning backbones available. These cloning backbones are empty vectors that can be modified to fit your needs. Each vector is available at research grade and is royalty and license free from research through commercial applications.
 
pALD-CV42 and pALD-CV77 are ideal for researchers who do not have a cloning backbone available. This construct set is appropriate for a wide range of applications. Researchers have also used the pALD-CV77 backbone sequence successfully in clinical trials.  

Aldevron-Cloning-Backbone-Benefits

 

Aldevron now offers pALD-CV42 [T7] and pALD-CV42 [T7-PolyA]. These cloning backbones contain the T7 promoter region for RNA production, with an option for encoded poly(A) available in one construct.

pALD-CV42-T7-and-pALD-CV42-T7-PolyA-graphic

pALD-CV42 [T7] Map_WPRE

pALD-CV42 [T7-PolyA] Map_WPRE

Request Sequence

Aldevron's cloning vectors are available as free samples. If you are interested, please fill out the Request Sample form.

Interested in rAAV Manufacturing? Check out rAAV specific ITR cloning vectors.

pALD-CV42 Quality Control Specifications

Appearance Clear and Colorless
Buffer TE
Concentration 1.0mg/mL +/- 10%
DNA Homogeneity Predominately Supercoiled
Endotoxin ≤ 100 EU/mg
Identity Confirmed Against DNA Ladder
Plasmid Identity Identical to Reference Sequence
ABS 260/280 Ratio Purity 1.80 – 2.00
Residual Host Genomic DNA ≤ 5%
Residual Host RNA Not Visible at 200 ng Load
Restriction Digest Matches Expected Restriction Pattern
Request a Complete CoA

pALD-CV77, pALD-CV42 [T7] and pALD-CV42 [T7-PolyA] Quality Control Specifications

Appearance Clear and Colorless
Buffer TE
Concentration 1.0mg/mL +/- 10%
DNA Homogeneity ≥ 80% Supercoiled
Endotoxin ≤ 100 EU/mg
Identity Size Confirmed Versus Supercoiled Marker
Plasmid Identity Identical to Reference Sequence
ABS 260/280 Ratio Purity 1.80 – 2.00
Residual Host Genomic DNA ≤ 5%
Residual Host RNA Not Visible at 200 ng Load
Restriction Digest Matches Expected Restriction Pattern
Request a Complete CoA

Commonly Asked Questions

Q: What are the main key features of this construct set?

A: Aldevron offers this construct set royalty free for research through commercial production. Complimentary samples are available for cloning and testing purposes.