Recombinant Protein Production

We develop strategies in E. coli, Insect and mammalian expression systems to produce proteins specific for your application. Our team can execute both technical transfer of your protocol or develop a customized process.

Expression in Bacterial Cells

Service Options	Details
E. coli Strains	Strains selected for each project, all common expression strains are available
Expression Optimization	SDS-PAGE or WB extracts (total, soluble and insoluble protein) multiple constructs, strains, media, and induction temperatures, 6 and 12 data points standard, optional micro affinity purification
E. coli Expression, Flask	60L per expression (15° - 40° C) and 5 runs per week
E. coli Expression, Fermenter	10 - 440 L
E. coli Expression, Fermenter	Custom fermentation development 4 X 5 L or 8 X 1 L in parallel

Protein Purification

Service Options	Details
Tech Transfer or Development	Reproduce client methods or develop de novo strategy
Chromatography	All common techniques including affinity, IEX, HIC, SEC AVANT 25 & 150 and AKTA Explorers
Scouting	Develop or refine methods by scouting >10 or more conditions per batch
Scale, Throughput	Microgram scale up to 50 g. Parallel purification options available for HT. Up to 90 samples per lot in batch mode, up to 36 samples per lot using column mode purification
Refolding, Dilution or Dialysis	Custom 24 condition dilution refolding screen up to 200 L scale dilution refold
Formulation Screen/Stability Study	96 condition formulation screen (buffer/excipient/detergent/salt) or custom. Monitored by turbidity, concentration, SEC-HPLC, target-specific activity assay. Data can be used for purification method development and long-term storage

Quality Control

Service Options	Details
PAGE	Denaturing, reducing, non-reducing, densitometry (Bio-Rad ChemiDoc XRS+)
Concentration	Dye binding assay, A280, RP-HPLC
Western Blot	Anti-Tag or Anti-Target, Colorimetric or chemiluminescence (ChemiDoc XRS+)
Analytical SEC	Standard or client-specific methods
Reverse Phase HPLC	Standard or client-specific methods, purity and / or concentration measurement
Mass Spec	Whole protein mass by MALDI or ESI, in-gel digest, LC-MS/MS, by qualified partner
Endotoxin	LAL Assay
Activity Assay	Absorbance, fluorescence, luminescence (Cuvette to 384-well plate)
ELISA	Anti-tag or anti-target, host cell protein, commercial or custom