pALD Lenti Products
Lentiviral vector plasmids
A solution from early research through commercial development
Aldevron provides pALD Lenti packaging plasmids through our partnership with OXGENE, who have optimized them for lentiviral vector production. They are immediately available at research grade, GMP-Source™, and cGMP. This will potentially reduce your cost and time to manufacture vectors for clinical trials.
The Aldevron pALD-Lenti system has several advantages:
Provided under license from:
We provide three packaging plasmids and an expression plasmid, including:
We can clone your gene of interest into the pALD-Lenti expression plasmid, replacing the EGFP sequence.
The system has been optimized and represents the state of the art in lentiviral vector production, including:
- Codon optimization
- Vector backbones minimized
- Minimization of homology with HIV/VSV
- Minimization of inter-cassette homology
In addition to the optimized design, lentiviral vectors produced with the pALD-Lenti system transfect cells with a higher infectious titer than commercially available kits.
- Download the label license for use of pALD Lenti products
- Download a non-confidential transfection protocol without plasmid ratio information
- Request access to sequences and confidential transfection protocol
Note: pALD-VSV-G products are classified by Aldevron under 1C353 on the Commerce Control List set forth in the US Export Administration Regulations (EAR), which is highly controlled for purposes of export outside the United States. If the product is requested to be shipped outside the US, an export license from US Dept. of Commerce’s Bureau of Industry and Security is required before the product may be shipped.
pALD-Lenti Plasmid Maps
|Clear and Colorless
|USP Sterility <71> *
|1.0 mg/mL +/- 10%
|≥ 80% Supercoiled
|≤ 100 EU/mg
|Confirmed Against DNA Ladder
|Identical to Reference Sequence
|ABS 260/280 Ratio Purity
|1.80 - 2.00
|Residual Host Genomic DNA via qPCR
|Residual Host Protein via BCA
|Residual Host RNA via SYBR Gold
|≤ 5% by Semi-Quantitative Densitometry
|Matches Expected Restriction Pattern
|Negative for the Presence of Mycoplasma
*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.