CRISPR Prime Editing Solutions

Precise substitutions, insertions, and deletions

Expand what's possible with genome editing

Aldevron, in partnership with IDT, offers Prime Editing (PE) Ultra mRNA designed to meet the demands of modern genome editing workflows.

Prime editing enables researchers to introduce targeted DNA substitutions, insertions, and deletions without requiring double-strand DNA breaks or donor DNA templates.

Designed to support the next generation of genome engineering, prime editing solutions combine Aldevron's optimized prime editor mRNA and Integrated DNA Technologies' (IDT) chemically modified pegRNAs, design tools, and technical expertise to accelerate precision editing experiments.

Performance Highlights

Looking for single-base editing? Explore our Base Editing solutions

Available Formats (Research Grade Only)

Enzymatically capped mRNA encoding PE Ultra Prime Editor (PE) in a eukaryotic expression context. Encodes an engineered S. pyogenes-derived nCas9 fused to an engineered reverse transcriptase. Contains 5' and 3' UTRs, Cap 1 structure, >100 nt poly(A) tail, nuclear localization sequences (NLS), and is fully N1-methylpseudouridine modified. Research grade mRNA manufactured by Aldevron and provided at 1 mg/mL in 1 mM sodium citrate buffer, pH 6.4.

Item No Description Purchase Option
3830-0020 PE Ultra mRNA, 20 µg Buy Now
3830-0100 PE Ultra mRNA, 100 µg Buy Now
3830-1 PE Ultra mRNA, 1 mg Buy Now

Design and order optimized Alt-R pegRNAs using IDT’s online design tool.

Looking for cGMP versions of PE Ultra mRNA? Contact us directly by filling out the form and a member of our team will reach out to you.

Product Details

Prime editing

Prime editing is a CRISPR-based genome editing modality that enables targeted DNA modifications without creating double-strand breaks or relying on donor DNA templates. Compared to traditional CRISPR-Cas9 editing, prime editing offers broader editing flexibility while reducing reliance on cellular DNA repair pathways

Prime editing utilizes:

This approach enables:

Unlike base editing, which is used to create specific nucleotide conversions, prime editing supports a broader range of genomic modifications. Click here for base editing solutions.

Why researchers choose prime editing

Precision editing for translational research

As genome editing programs move closer to clinical application, researchers need editing technologies that can deliver precise genomic changes while minimizing unintended edits. Prime editing enables targeted substitutions, insertions, and deletions without requiring double-strand DNA breaks or donor DNA templates, offering a powerful approach for applications where precision and control are critical.

Streamline the path from discovery to translation

The combined IDT and Aldevron platform provides access to optimized prime editing reagents, highly modified guide RNAs, design support, and manufacturing expertise to help researchers move from early discovery through advanced translational research. By providing continuity across genome editing workflows, Aldevron helps simplify development while supporting future clinical ambitions.

Confidence through engineered performance

Aldevron prime editing solutions include an optimized prime editor mRNA and IDT's chemically modified pegRNAs designed to improve editing success and stability. Together with a free online design tool, these solutions help researchers spend less time troubleshooting complex workflows and more time generating actionable data.

Not sure which editing approach is right for your application?

Compare base and prime editing to find the best fit

Product Data

Comparison of editing efficiency of Aldevron’s PE Ultra mRNA to commercially available PEmax

Figure 1. Editing efficiency of PE Ultra and PEmax in HEK293 cells. PE Ultra and PEmax mRNA and Alt-R pegRNA (6 µM) targeting a broad panel of sites (all 6 bp insertions) were delivered by nucleofection into HEK293 cells using the Lonza Nucleofection™ System. Genomic DNA (gDNA) was extracted 72 hours post-nucleofection and editing was assessed by NGS.

PE Ultra mRNA and Alt-R pegRNA efficiently generate different mutation types across various immortal and primary cell lines

Figure 2. Editing efficiency of PE Ultra after nucleofection into multiple cell lines. PE Ultra mRNA, 6 uM Alt-R pegRNA and 2 uM nsgRNA targeting ACAT2, ADPGK, AK2, AGPAT1, FANCF and DMNT1 were delivered by nucleofection into HEK293 cells (A), K562 cells (B), iPSCs (C), and T cells (D) using the Lonza Nucleofection™ System. Genomic DNA (gDNA) was extracted 72 hours post-nucleofection and editing was assessed by NGS. Edit types included insertions (3 bp: FANCF and DMNT1, 6 bp: ACAT2, ADPGK, AK2 and AGPAT1), deletions (all 3 bp) and SNP (all 1 bp). The data is the average of the 6 sites for each edit type, N=3.

Alt-R pegRNAs are modified and optimized for superior editing in primary cells via LNP delivery

Figure 3. Alt-R pegRNAs are modified and optimized for superior editing in primary cells via LNP delivery. PE Ultra mRNA, Alt-R pegRNA, and ngRNAs were encapsulated into lipid nanoparticles (LNP) using the GenVoy-ILM™ T Cell Kit on the NanoAssemblr™ Spark instrument, following manufacturer’s instructions. Briefly, RNA working solutions were prepared in the supplied formulation buffer with mRNA, pegRNA, and nsgRNA mixed at a 6:5:1 ratio respectively by RNA weight. The lipid phase and RNA solution were then combined in the Spark instrument. Primary T cells were thawed and activated 72 hours prior to LNP addition. Cells were seeded at 0.5 x 105 cells/well (iPSC, A), and 0.1 x 106 cells/well (Primary T cells, B), followed by direct addition of the LNPs. Genomic DNA (gDNA) was extracted 72 hours post-delivery and editing was assessed by NGS. pegRNAs were designed to introduce a 6 bp insertion at ADPGK. End modified pegRNA contains 3x 2’O-Methyl modifications and 3x phosphorothioate bonds on the 5’ and 3’ ends. (N=3)

Frequently Asked Questions

What products do I need to buy to perform prime editing?
Prime editor (PE) mRNA and prime editing prime guide RNA (pegRNA) are the basic components of any prime editing experiment. PegRNA can be purchased from IDT.
What components should be included when designing a prime editing guide RNA (pegRNA) for prime editing?
The key components of a pegRNA are the spacer, scaffold, primer binding site (PBS), and reverse transcription template (RTT).
What are the spacer, scaffold, primer binding site (PBS) and reverse transcription template (RTT) of a pegRNA?
The spacer is a 20-nt sequence that guides the prime editor to the target DNA site through base pairing. The scaffold forms the RNA structure required for prime editor complex assembly. The primer binding site (PBS) anneals to the nicked DNA strand and initiates reverse transcription. The reverse transcription template (RTT) contains the desired edit and serves as the template for reverse transcriptase to incorporate the edited sequence into the genome.
What is the purpose of a nicking guide (ngRNA)?
The purpose of a nicking guide (ngRNA) is to introduce a nick in the non-edited DNA strand, encouraging cellular repair pathways to use the edited strand as a template for repair and thereby increasing prime editing efficiency.
How do I choose the spacer, reverse transcription template (RTT), and primer binding site (PBS)?
IDT offers a free configurator tool that generates suitable pegRNA sequences for screening based on your desired edit and target edit site.
How does prime editing differ from base editing or HDR-based CRISPR editing?
Base editing is the preferred approach for rapid and efficient correction of compatible single-base mutations, specifically C→T or A→G conversions. Prime editing is better suited for precise modifications beyond these transitions, including other base substitutions and small insertions or deletions, while avoiding the creation of double-strand DNA breaks. HDR-mediated CRISPR editing can also achieve substitutions, insertions, deletions, and even larger sequence replacements. However, HDR-based approaches generally exhibit lower editing efficiency and may carry a higher risk of off-target effects and genotoxicity.
Which delivery method can be used for delivery of prime editor and pegRNA?
Our prime editing products are compatible with electroporation and LNP delivery. Additional delivery methods may be compatible, but we have not validated such methods.
What kind of edits can be performed with prime editing?
Prime editing is particularly well suited for base substitutions that are not accessible with base editors, as well as small insertions and deletions.
Do you have a protocol for prime editing?
We offer a prime editing protocol for electroporation workflows. This protocol can be found here.

Need Help?

Have questions for our CRISPR experts or need a custom solution? Your time is valuable and we’ll prioritize your inquiry. Fill out the form to provide brief information about your project, and we’ll be in touch to discuss it as soon as possible.

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