CRISPR Base Editing Solutions
Precise and efficient adenine base editing without double-stranded breaks
Designed for precise genome editing
Aldevron, in partnership with IDT, offers adenine base editor (ABE) mRNAs designed to meet the demands of modern genome editing workflows.
Base editing enables targeted nucleotide conversion without creating double-strand DNA breaks or requiring donor DNA templates. Designed for researchers developing next-generation cell and gene therapies, Aldevron base editing solutions combine stocked ABE mRNAs, highly modified guide RNAs, targeting flexibility through multiple PAM options, and technical support to help streamline precision editing workflows.
Performance Highlights
- Enable precise A-to-G genome edits without double-strand DNA breaks, helping reduce genotoxicity concerns in translational research applications
- Expand your editable target regions with multiple PAM-compatible editor variants and high-specificity options designed to address challenging genomic loci.
- ABE mRNA screening kit to determine the best approach for your experiments
- Improve editing success with stocked base editor mRNAs, optimized sgRNA XT chemistry, and expert support
Looking for cGMP versions of Adenine Base Editor mRNA? Contact us directly by filling out the form and a member of our team will reach out to you.
Available Formats (Research Grade Only)
Base Editor mRNA enzymes
| Item No | Description | Purchase Option |
| 3840-0020 | ABE8e nCas9 mRNA, RG, 20 µg | Buy Now |
| 3840-0100 | ABE8e nCas9 mRNA, RG, 100 µg | Buy Now |
| 3840-1 | ABE8e nCas9 mRNA, RG, 1 mg | Buy Now |
| 3841-0020 | ABE8e nSpG mRNA, RG, 20 µg | Buy Now |
| 3841-0100 | ABE8e nSpG mRNA, RG, 100 µg | Buy Now |
| 3841-1 | ABE8e nSpG mRNA, RG, 1 mg | Buy Now |
| 3842-0020 | ABE8e nSPRY mRNA, RG, 20 µg | Buy Now |
| 3842-0100 | ABE8e nSPRY mRNA, RG, 100 µg | Buy Now |
| 3842-1 | ABE8e nSPRY mRNA, RG, 1 mg | Buy Now |
| 3843-0020 | ABE8e-V106W nCas9 mRNA, RG, 20 µg | Buy Now |
| 3843-0100 | ABE8e-V106W nCas9 mRNA, RG, 100 µg | Buy Now |
| 3843-1 | ABE8e-V106W nCas9 mRNA, RG, 1 mg | Buy Now |
| 3844-0020 | ABE8e-V106W nSpG mRNA, RG, 20 µg | Buy Now |
| 3844-0100 | ABE8e-V106W nSpG mRNA, RG, 100 µg | Buy Now |
| 3844-1 | ABE8e-V106W nSpG mRNA, RG, 1 mg | Buy Now |
| 3845-0020 | ABE8e-V106W nSPRY mRNA, RG, 20 µg | Buy Now |
| 3845-0100 | ABE8e-V106W nSPRY mRNA, RG, 100 µg | Buy Now |
| 3845-1 | ABE8e-V106W nSPRY mRNA, RG, 1 mg | Buy Now |
Alt-R™ Cas9 sgRNA XT
Optimized guide RNAs designed for mRNA- and LNP-based genome editing workflows.
What is V106W? ABE8e V106W is a published adenine base editor variant1 designed to reduce bystander editing while maintaining strong on-target editing activity. It is well suited for applications where increased editing precision and a narrower editing profile are desired.
Product Details
Stocked Base Editor mRNAs
Aldevron offers six stocked adenine base editor (ABE) mRNAs available in 20 μg, 100 μg, and 1 mg formats.
| Product | PAM |
| WT ABE8e nCas9 | NGG |
| WT ABE8e nSpG | NGN |
| WT ABE8e nSPRY | NRN |
| ABE8e V106W nCas9 | NGG |
| ABE8e V106W nSpG | NGN |
| ABE8e V106W nSPRY | NRN |
Designed for Flexibility
- Wild type and high-specificity V106W variants1 available
- Multiple PAM options expand editable genomic regions
- Stocked mRNAs ship in as little as 1 business day
Integrated DNA Technologies’ (IDT) Alt-R™ Cas9 sgRNA XT
Optimized guide RNAs designed for mRNA- and LNP-based genome editing workflows.
Features
- Available from 2–100 nmol
- Desalt and HPLC purification options
- Increased 2'-O-methyl content for stabilization
- Designed specifically for mRNA and LNP delivery workflows
Not sure which editing approach is right for your application?
Compare base and prime editing to find the best fit
Product Data
On target vs bystander conversion of various ABE editors in HEK293 cells
Figure 1. Comparison of maximal window and bystander editing for ABE8e variants. Maximum on-target A→G editing at one base within the canonical ABE8e editing window (protospacer positions 4-12) was compared with the highest observed bystander edit outside the window for ABE8e-WT and ABE8e-V106W paired with nCas9, nSpG, or nSPRY across representative genomic loci in HEK293 cells. Cells were nucleofected with 1,000ng base editor mRNA and 2 µM gRNA using the Lonza nucleofection system. Bars represent mean ± SEM (N = 3 biological replicates). ABE8e-V106W maintained high on-target editing while reducing bystander editing relative to ABE8e-WT at multiple loci.
Base conversion efficiency across various immortal and primary cell lines
Figure 2. Target adenine editing efficiency across cell types for ABE8e-Cas variant combinations. Target adenine conversion at the indicated genomic loci was measured in HEK293, iPSC, K562, and primary T cells using ABE8e editors paired with nCas9, nSpG, or nSPRY. Data from ABE8e-WT and ABE8e-V106W were combined due to comparable on-target editing efficiencies and are presented as mean ± SEM (N = 6 biological replicates; 3 WT and 3 V106W). Cells were nucleofected using the Lonza nucleofection system with 1,000ng of base editor mRNA in HEK293, K562, and primary T cells or 500ng in iPSCs. gRNA was delivered at 2 µM in HEK293, K562, and iPSCs and 4 µM in primary T cells. High levels of target base conversion were observed across diverse cell types and Cas variants.
ABE conversion efficiency in Primary T cells via LNP delivery
Figure 3. Target base conversion following LNP delivery of ABE8e editor systems. Maximum target adenine editing at the indicated genomic loci was measured in primary T cells following lipid nanoparticle delivery of ABE8e-WT paired with nCas9, nSpG, or nSPRY. Cells were treated with 2 µg base editor mRNA and 2 µg gRNA per million cells. Data are shown as mean ± SEM (N = 3 biological replicates). Efficient target base conversion was observed across all loci and Cas variants tested.
Target base conversion efficiency under singleplex or multiplex conditions
Figure 4. Target adenine conversion under singleplex and multiplex editing conditions. Target adenine conversion was evaluated at four genomic sites in HEK293 cells following delivery of ABE8e-WT or ABE8e-V106W paired with nCas9 under singleplex or 4-plex pooled gRNA conditions. Cells were nucleofected using the Lonza nucleofection system with 1,000 ng base editor mRNA and 2 µM gRNA for singleplex editing or 2 µM of each gRNA for 4-plex pooled editing. Data are presented as mean ± SEM from three biological replicates (N = 3). Comparable editing efficiencies were observed between singleplex and multiplex conditions, demonstrating robust multiplex editing performance across target sites.
Frequently Asked Questions
What type of DNA base editor is fused to the BE Cas9?
We offer an mRNA version of the Adenine Base Editor 8e (ABE8e), a highly active adenine base editor described by Richter et al. (2020).
The editor enables A-to-G•C base conversion without generating double-stranded DNA breaks.
The editor enables A-to-G•C base conversion without generating double-stranded DNA breaks.
What products do I need to buy with my base editor?
A base editing guide RNA (gRNA) is required to direct the base editor to the desired genomic target. At a minimum, a base editing experiment requires the ABE8e mRNA base editor and a compatible Cas9 gRNA.
Depending on your workflow, additional reagents for delivery and downstream analysis may also be required. Cas9 gRNA can be purchased from IDT.
Depending on your workflow, additional reagents for delivery and downstream analysis may also be required. Cas9 gRNA can be purchased from IDT.
Do you have a protocol for base editing using an mRNA?
Yes. We provide a base editing protocol for electroporation-based workflows. The protocol is available here.
What is the purpose/advantage of using V106W Base Editor (BE) over WT BE?
The V106W variant was designed to improve editing specificity relative to the wild type (WT) mRNA base editor1.
In many applications, it reduces bystander editing while maintaining robust on-target editing activity, making it a useful option when precise editing outcomes are desired.
In many applications, it reduces bystander editing while maintaining robust on-target editing activity, making it a useful option when precise editing outcomes are desired.
Should I choose a Base Editor or Prime Editor for my target of interest?
The optimal approach depends on the desired edit. Base editing is often the preferred option for direct A-to-G single-base substitutions because it typically provides higher editing efficiency.
Prime editing may be a better choice when precise sequence changes are required, such as edits that cannot be achieved with base editing or applications where bystander edits are not acceptable.
Prime editing may be a better choice when precise sequence changes are required, such as edits that cannot be achieved with base editing or applications where bystander edits are not acceptable.
Need Help?
Have questions for our CRISPR experts or need a custom solution? Your time is valuable and we’ll prioritize your inquiry. Fill out the form to provide brief information about your project, and we’ll be in touch to discuss it as soon as possible.
References
- Gaudelli et al. (2020), Directed evolution of adenine base editors with increased activity and therapeutic application (Nature Biotechnology).