In Stock, Royalty-Free, Packaging Plasmid Solution for Recombinant AAV Manufacturing

pALD-AAV Rep/Cap Plasmids

Recombinant AAV (rAAV) manufacturing is composed of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.

Aldevron's pALD-AAV rep/cap plasmids have several advantages:

• Royalty free, including for commercial products
• Kanamycin resistance
• Consistent, reliable supply
• Immediately available at research grade

With Aldevron's rep/cap plasmids, clients do not need to manufacture a custom batch as Aldevron maintains each as an in-stock product, reducing timeline and cost.

Each rep/cap plasmid contains the gene sequence for AAV serotype 2 replication (rep) protein paired with either serotype 2, 5 or 6 capsid (cap) protein respectively. Use of AAV serotype 2 rep proteins in combination with other serotypes for cap proteins is referred to as pseudotyping which provides enhanced and refined tissue tropism in some applications.

Bulk quantities and samples are available upon request.