In Stock, Royalty-Free, Packaging Plasmid Solution for Recombinant AAV Manufacturing
pALD-AAV Rep/Cap Plasmids
Recombinant AAV (rAAV) manufacturing is composed of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.
Aldevron's pALD-AAV rep/cap plasmids have several advantages:
- Royalty free, including for commercial products
- Kanamycin resistance
- Consistent, reliable supply
- Immediately available at research grade
With Aldevron's rep/cap plasmids, clients do not need to manufacture a custom batch as Aldevron maintains each as an in-stock product, reducing timeline and cost.
Each rep/cap plasmid contains the gene sequence for AAV serotype 2 replication (rep) protein paired with either serotype 2, 5 or 6 capsid (cap) protein respectively. Use of AAV serotype 2 rep proteins in combination with other serotypes for cap proteins is referred to as pseudotyping which provides enhanced and refined tissue tropism in some applications.
|Plasmid||Rep Serotype||Cap Serotype||Pack Size|
Bulk quantities and samples are available upon request.
5057-10 10 mg Pricing
5058-10 10 mg Pricing
5059-10 10 mg Pricing
Rep/Cap Plasmids Quality Control Specifications
|Appearance||Clear and Colorless|
|USP Sterility <71> *||No Growth|
|Concentration||1.0 mg/mL +/- 10%|
|DNA Homogeneity||≥ 80% Supercoiled|
|Endotoxin||≤ 100 EU/mg|
|Identity||Confirmed Against DNA Ladder|
|Plasmid Identity||Identical to Reference Sequence|
|ABS 260/280 Ratio Purity||1.80 - 2.00|
|Residual Host Genomic DNA via qPCR||≤ 5%|
|Residual Host Protein via BCA||≤ 2%|
|Residual Host RNA via SYBR Gold||≤ 5% by Semi-Quantitative Densitometry|
|Restriction Digest||Matches Expected Restriction Pattern|
|Mycoplasma Contamination||Negative for the Presence of Mycoplasma|
*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.Request a Complete CoA