pALD-ITR Plasmids

In Stock, Royalty-Free, Expression Plasmid Solutions for Recombinant AAV Manufacturing

Recombinant AAV (rAAV) manufacturing is comprised of four main parts which include a rep/cap plasmid, a helper plasmid, an expression plasmid, and a viral packaging cell line. Triple transfection of the three plasmids into HEK293 cells is the most frequently used rAAV production method.

Aldevron now offers reporter plasmids and cloning vectors specifically for rAAV manufacturing.

pALD-ITR GFP Reporters:

Aldevron's pALD-ITR GFP reporter plasmids have several advantages:

  • Royalty free for research use
  • Kanamycin resistance
  • Available with and without WPRE
  • Consistent, reliable supply
  • Immediately available at research grade

pALD-ITR Cloning Vectors:

Aldevron's pALD-ITR cloning vectors have several advantages:

  • Royalty free for research through commercial use
  • Kanamycin resistance
  • Available with and without WPRE
  • Available off the shelf for cloning and gene synthesis
  • Immediately available at research grade

Aldevron's pALD-ITR GFP reporter plasmids and cloning vectors are available as free samples. If you are interested, please fill out the request sample form.

Description Cat # Price Order

pALD-ITR-GFP, RG, 10 mg

 5062-10 pALD-ITR-GFP
5062-10 pALD-ITR-GFP 10mg Pricing
Quantity:
Size:

pALD-ITR-WPRE-GFP, RG, 10 mg

 5069-10 pALD-ITR-WPRE-GFP
5069-10 pALD-ITR-WPRE-GFP 10mg Pricing
Quantity:
Size:

pALD-ITR GFP Reporters Quality Control Specifications

Appearance Clear and Colorless
Buffer TE
Bioburden No growth in 48 hours at 37 +/- 2 C
Concentration 1.0mg/mL +/- 10%
DNA Homogeneity ≥80% Supercoiled
Endotoxin ≤100 EU/mg
Identity Confirmed Against DNA Ladder
Plasmid Identity Identical to Reference Sequence
ABS 260/280 Ratio Purity 1.80 – 2.00
Residual Host Genomic DNA via qPCR ≤5%
Residual Host Protein via BCA ≤2%
Residual Host RNA via SYBR Gold ≤5% by Semi-Quantitative Densitometry
Restriction Digest Matches Expected Restriction Pattern
Mycoplasma Contamination Negative for the Presence of Mycoplasma
Request a Complete CoA

pALD-ITR Cloning Vectors Quality Control Specifications

Appearance Clear and Colorless
Buffer TE
Concentration 1.0mg/mL +/- 10%
DNA Homogeneity ≥80% Supercoiled
Endotoxin ≤100 EU/mg
Identity Confirmed Against DNA Ladder
Plasmid Identity Identical to Reference Sequence
ABS 260/280 Ratio Purity 1.80 – 2.00
Residual Host Genomic DNA ≤5%
Residual Host RNA Not Visible at 200 ng Load
Restriction Digest Matches Expected Restriction Pattern
Request a Complete CoA