pALD-HELP for rAAV Production

In Stock, Royalty-Free, Helper Plasmid Solution for Recombinant AAV Manufacturing

Triple transfection is the most common method for rAAV production and does not require co-infection with adenovirus; a helper plasmid provides adenoviral genes. Aldevron's pALD-HELP has several advantages:

  • Immediately available at research grade
  • Royalty free, including for commercial products
  • Replaces any rAAV helper plasmid
  • Kanamycin resistance
  • Consistent, reliable supply

With Aldevron's pALD-HELP, clients do not need to manufacture a custom batch of helper plasmid because Aldevron maintains it as an in-stock product, providing a high-quality and consistent source, helping reduce timelines and cost.

pALD-HELP Common@2x

 

Available Pack Sizes

Product  Free Sample Research Grade GMP-Source® cGMP
pALD-HELP 100 μg 10 mg 10 mg Custom Request

 

pALD-HELP, pALD-AAV Rep/Cap Infectious Titer Data

pALD-Help Data@2x

Infectious titer data for rAAV vector production in adherent HEK293T cells using pALD-HELP and pALD-AAV rep/cap plasmids as compared to commercially available rep/cap plasmids.

 

pALD-HELP Quality Control Specifications

Appearance Clear and Colorless
Buffer TE
USP Sterility <71> * No Growth
Concentration 1.0 mg/mL +/- 10%
DNA Homogeneity ≥ 80% Supercoiled
Endotoxin ≤ 100 EU/mg
Identity Confirmed Against DNA Ladder
Plasmid Identity Identical to Reference Sequence
ABS 260/280 Ratio Purity 1.80 - 2.00
Residual Host Genomic DNA via qPCR ≤ 5%
Residual Host Protein via BCA ≤ 2%
Residual Host RNA via SYBR Gold ≤ 5% by Semi-Quantitative Densitometry
Restriction Digest Matches Expected Restriction Pattern
Mycoplasma Contamination Negative for the Presence of Mycoplasma

*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.