gWiz™ High Expression Reporter Plasmids

The gWiz plasmids are designed for the highest level of gene expression in a wide range of mammalian cells and tissues. They contain a proprietary modified promoter, followed by intron A from the CMV immediate early gene, and a high-efficiency artificial transcription terminator. gWiz expression vectors are constructed using a plasmid backbone that has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.

Aldevron's exclusive fermentation technology and DNA purification process are used to produce ultrapure, milligram amounts of the gWiz™ plasmids. These ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Each gWiz plasmid is delivered in the buffer of your choice with delivery time of approximately 24 to 48 hours. Volume discounts are available. Common features of the gWiz plasmids include:

  • In vitro or in vivo gene expression
  • CMV IE promoter
  • Intron A
  • Artificial transcription terminator
  • Kanamycin resistance

gWiz high expression reporter plasmids available from Aldevron.

Vector Cat # Price Order
gWiz-GFP

MAP

QUANTITY PRICING
Cat# 5007 Only
Transgene Green Fluorescent Protein
Plasmid Size 5757 bp
Notes GFP expression in mammalian cells and tissues
5006
5007
- 5 mg
- 10 mg
Quantity:
Size:
gWiz-Luc

MAP

QUANTITY PRICING
Cat# 5001 Only
Transgene Luciferase
Plasmid Size 6732 bp
Notes Luc expression in mammalian cells and tissue
5000
5001
- 5 mg
- 10 mg
Quantity:
Size:
gWiz-blank

MAP

QUANTITY PRICING
Cat# 5009 Only
Transgene none
Plasmid Size 5100 bp
Notes Multiple cloning site for inserting gene of interest; Expression in mammalian cells and tissue
5008
5009
- 5 mg
- 10 mg
Quantity:
Size:

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gWiz Quality Control Specifications

Appearance Clear and colorless
Buffer Water
Concentration 5.0 mg/mL +/- 10%
DNA Homogeneity Predominantly supercoiled
Endotoxin ≤ 100 EU/mg
Identity Confirmed Against DNA Ladder
ABS 260/280 Ratio Purity 1.80 - 2.00
Residual Host Genomic DNA ≤ 5%
Residual Host RNA Not visible at 200 ng load
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