The gWiz plasmids are designed for the highest level of gene expression in a wide range of mammalian cells and tissues. They contain a proprietary modified promoter, followed by intron A from the CMV immediate early gene, and a high-efficiency artificial transcription terminator. gWiz expression vectors are constructed using a plasmid backbone that has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.
Aldevron's exclusive fermentation technology and DNA purification process are used to produce ultrapure, milligram amounts of the gWiz™ plasmids. These ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Each gWiz plasmid is delivered in the buffer of your choice with delivery time of approximately 24 to 48 hours. Volume discounts are available. Common features of the gWiz plasmids include:
- In vitro or in vivo gene expression
- CMV IE promoter
- Intron A
- Artificial transcription terminator
- Kanamycin resistance
gWiz Quality Control Specifications
| Appearance | Clear and colorless |
| Buffer | Water |
| Concentration | 5.0 mg/mL +/- 10% |
| DNA Homogeneity | Predominantly supercoiled |
| Endotoxin | ≤ 100 EU/mg |
| Identity | Confirmed Against DNA Ladder |
| ABS 260/280 Ratio Purity | 1.80 - 2.00 |
| Residual Host Genomic DNA | ≤ 5% |
| Residual Host RNA | Not visible at 200 ng load |
