SpyFiTM Cas9 Nuclease

Reduced off-target activity without loss of editing efficiency

From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research Grade, GMP-Source® and GMP levels.

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SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.

Functional Performance

  • Reduced off-target effects
  • Ready to use for electroporation, transfections or injection methods
  • 10 mg/mL formulation for ease of use

Manufacturing and Quality Control

  • Research grade to GMP
  • Consistent activity and purity across all lots

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Aldevron’s SpyFi Cas9 Nuclease is sold under license of patents and/or patents pending from Integrated DNA Technologies, Inc. (IDT). Data shown above was generated in collaboration with IDT.

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To request your CoA, please provide your contact information. We do not share your information with any third parties.

SpyFi Quality Control Specifications

Purity, RP-HPLC  ≥95%
Purity, SDS-PAGE  ≥95%
Aggregation  ≤5% aggregate
Concentration 10.0 mg/mL +/- 10%
Endotoxin  ≤ 10 EU/mg
Residual DNase  < LOQ
Residual RNase  < LOQ
Appearance  Clear, colorless
Residual Host Protein  ≤ 200 ng/mL
Residual Host GenomicDNA  < 1% (w/w)
Activity Assay, in vitro  ≥80% of uncut reference DNA converted to cleavage products
Bioburden  < 2 CFU/Sample
Mycoplasma  Negative for the presence of Mycoplasma

 

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