Reduced off-target activity without loss of editing efficiency
From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research Grade, GMP-Source® and GMP levels.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
- Reduced off-target effects
- Ready to use for electroporation, transfections or injection methods
- 10 mg/mL formulation for ease of use
Manufacturing and Quality Control
- Research grade to GMP
- Consistent activity and purity across all lots
Aldevron’s SpyFi Cas9 Nuclease is sold under license of patents and/or patents pending from Integrated DNA Technologies, Inc. (IDT). Data shown above was generated in collaboration with IDT.
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Purity, RP-HPLC | ≥95% |
Purity, SDS-PAGE | ≥95% |
Aggregation | ≤5% aggregate |
Concentration | 10.0 mg/mL +/- 10% |
Endotoxin | ≤ 10 EU/mg |
Residual DNase | < LOQ |
Residual RNase | < LOQ |
Appearance | Clear, colorless |
Residual Host Protein | ≤ 200 ng/mL |
Residual Host GenomicDNA | < 1% (w/w) |
Activity Assay, in vitro | ≥80% of uncut reference DNA converted to cleavage products |
Bioburden | < 2 CFU/Sample |
Mycoplasma | Negative for the presence of Mycoplasma |
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