Custom Enzymes for Gene Editing
CRISPR-associated Nucleases
Aldevron offers custom manufacturing of CRISPR/Cas9 nucleases, including SpCas9 variants, SaCas9, dCas9 fusions and nickases. Custom variations of an existing Aldevron CRISPR nuclease are also available, including custom concentration, formulation or QC testing. Our manufacturing scale ranges from 10 mg to multi-gram lots.
Custom nucleases are supported by robust, scalable manufacturing protocols, including the capability of internally transferring from research grade to a cGMP facility. We also offer in-stock Research Grade, GMP-Source® and GMP CRISPR-associated nucleases in standard formulations for immediate delivery. These include SpyFiTM Cas9 Nuclease, SpCas9 and AsCpf1.
- Download our CRISPR/Cas9 Product Guide (PDF)
- Contact us to learn more about our custom manufacturing options
- Order in-stock CRISPR/Cas9 nucleases
Enzymes for RNA Production
Our in vitro transcription (IVT) enzymes are qualified both by activity assays and functionally for RNA synthesis and can be custom manufactured to meet your specific project requirements from milligram to multigram scale. View the table below for a summary of our IVT enzymes.
Product Name | Product Description |
---|---|
T7 RNA Polymerase |
T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter. |
Inorganic Pyrophosphatase |
Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate. |
Ribonuclease Inhibitor |
Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C. |
DNase I |
DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity. |
Guanylyltransferase |
Catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps: |
2’-O-Methyltransferase |
2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes |
Poly(A) Polymerase |
Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner. |