pALD-HELP for rAAV Production
In Stock, Royalty-Free, Helper Plasmid Solution for Recombinant AAV Manufacturing
Triple transfection is the most common method for rAAV production and does not require co-infection with adenovirus; a helper plasmid provides adenoviral genes. Aldevron's pALD-HELP has several advantages:
- Immediately available at research grade and GMP-Source®
- Royalty free, including for commercial products
- Replaces any rAAV helper plasmid
- Kanamycin resistance
- Consistent, reliable supply
With Aldevron's pALD-HELP, clients do not need to manufacture a custom batch of helper plasmid because Aldevron maintains it as an in-stock product, providing a high-quality and consistent source, helping reduce timelines and cost.
Infectious titer data for rAAV vector production in adherent HEK293T cells using pALD-HELP and pALD-AAV rep/cap plasmids as compared to commercially available rep/cap plasmids.
Additional discounts available for quantities > 5 vials.
|Clear and Colorless
|USP Sterility <71> *
|1.0 mg/mL +/- 10%
|≥ 80% Supercoiled
|≤ 100 EU/mg
|Confirmed Against DNA Ladder
|Identical to Reference Sequence
|ABS 260/280 Ratio Purity
|1.80 - 2.00
|Residual Host Genomic DNA via qPCR
|Residual Host Protein via BCA
|Residual Host RNA via SYBR Gold
|≤ 5% by Semi-Quantitative Densitometry
|Matches Expected Restriction Pattern
|Negative for the Presence of Mycoplasma
*Sterility testing is not available for research grade material. Bioburden by direct inoculation with a specification of no growth after 48 hours is performed at the research grade level.