Reporter Plasmids

In Stock Reporter Plasmids

Aldevron, your trusted partner since 1998, has several reporter plasmids available.

pALD-CV77-Luciferase Reporter

Consistent with the pALD-CV77 cloning backbone, Aldevron offers a pre-made reporter construct, pALD-CV77-Luciferase, which has several advantages:

Commercially available sequence for research through clinical use
Kanamycin resistance
Consistent, reliable supply
Immediately available at research grade
Available in 100ug free sample vials, 5mg vials and 10mg vials, research grade only

Request Sequence

gWiz Reporter Plasmids

The gWiz plasmids are designed for a wide range of mammalian cells and tissues. They contain a modified promoter, followed by intron A from the CMV immediate early gene, and an artificial transcription terminator. These ready-to-use plasmids can be applied directly to most in vitro and in vivo applications.

Each gWiz plasmid is ready to ship stocked at 5mg/mL in water enabling you to easily adjust to the buffer of your choice. Common features of the gWiz plasmids include:

In vitro or in vivo gene expression
CMV IE promoter
Intron A
Artificial transcription terminator
Kanamycin resistance
Available in 5mg and 10mg vials, research grade only

Quality & Ordering Information

pALD-CV77 Luciferase and gWiz Quality Control Specifications

	pALD-CV77-Luciferase	gWiz Reporter Plasmids
Appearance	Clear and Colorless	Clear and colorless
Buffer	TE	Water
Bioburden	No growth in 48 hours at 37 +/- 2 C	N/A
Concentration	1.0mg/mL +/- 10%	5.0 mg/mL +/- 10%
DNA Homogeneity	≥80% Supercoiled	Predominantly supercoiled
Endotoxin	≤100 EU/mg	≤ 100 EU/mg
Identity	Confirmed Against DNA Ladder	Confirmed Against DNA Ladder
Plasmid Identity	Identical to Reference Sequence	N/A
ABS 260/280 Ratio Purity	1.80 – 2.00	1.80 - 2.00
Residual Host Genomic DNA via qPCR	≤5%	≤ 5%
Residual Host Protein via BCA	≤2%	Not visible at 200 ng load
Residual Host RNA via SYBR Gold	≤5% by Semi-Quantitative Densitometry	N/A
Restriction Digest	Matches Expected Restriction Pattern	N/A
Mycoplasma Contamination	Negative for the Presence of Mycoplasma	N/A
Residual Host RNA	N/A	Not visible at 200 ng load

<table class="table_order"><thead><tr><th>Description</th><th>Cat #</th><th>Price</th><th>Order</th></tr></thead><tbody><tr><td><p><strong class="item_title">pALD-CV77-Luciferase 10 mg</strong></p></td><td>5078-10</td><td><div class="pricetable mmg-collapsible"><strong class="module-title">pALD-CV77-Luciferase 10 mg Pricing</strong><div id="pricetable_112851" class="content" style="display:none"> <table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody>

<tr>

</tr>

<tr>

</tr>

<tr>

</tr>

<tr>

</tr>

</tbody></table></div></div></td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/reporters/traditional-pdna-reporters" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" style="width:75px" name="buyid"><option value="112850">5 mg</option></select><br><button class="addtocart" type="submit">Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-GFP</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1594&c=606687&h=4f1ad11f91d7b22e1c9a" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5006/5007 gWiz-GFP"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56373" class="content" style="display:none">Cat# 5007 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td><td>$1100.00</td></tr><tr><td>2</td><td>$940.00</td></tr><tr><td>3</td><td>$940.00</td></tr><tr><td>4</td><td>$940.00</td></tr><tr><td>5+</td><td>$810.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Green Fluorescent Protein</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5757 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>GFP expression in mammalian cells and tissues</td></tr></tbody></table></td><td>5006<br>5007</td><td><span class="price" id="price_56482">$600.00</span>- 5 mg<br><span class="price" id="price_56373">$1,100.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56482">5 mg</option><option value="56373">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5006/5007 gWiz-GFP",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-Luc</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=706&c=606687&h=38863950c295c63ad752" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5000/5001 gWiz-Luc"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56370" class="content" style="display:none">Cat# 5001 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td> <td>$1100.00</td></tr><tr><td>2</td><td>$940.00</td></tr><tr><td>3</td><td>$940.00</td></tr><tr><td>4</td><td>$940.00</td></tr><tr><td>5+</td><td>$810.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>Luciferase</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>6732 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Luc expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5000<br>5001</td><td><span class="price" id="price_56479">$600.00</span>- 5 mg<br><span class="price" id="price_56370">$1,100.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/dnas/gwiz" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56479">5 mg</option><option value="56370">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5000/5001 gWiz-Luc",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr><tr><td class="labelcell"><strong class="item_title">gWiz-blank</strong><p><a href="https://606687.secure.netsuite.com/core/media/media.nl?id=1592&c=606687&h=9c668159252a11acd146" target="_blank" class="button zoom-map cboxElement" rel="noopener" onclick='_gaq.push(["_trackEvent","Button","Map","5008/5009 gWiz-blank"])'>MAP</a></p><div class="pricetable mmg-collapsible"><strong class="module-title">QUANTITY PRICING</strong><div id="pricetable_56374" class="content" style="display:none">Cat# 5009 Only<table><thead><tr><th nowrap="">Quantity</th><th nowrap="">Price (each)</th></tr></thead><tbody><tr><td>1</td> <td>$1100.00</td></tr><tr><td>2</td><td>$940.00</td></tr><tr><td>3</td><td>$940.00</td></tr><tr><td>4</td><td>$940.00</td></tr><tr><td>5+</td><td>$810.00</td></tr></tbody></table></div></div><table class="table_orderNested"><tbody><tr><td><span class="info_label">Transgene</span></td><td>none</td></tr><tr><td><span class="info_label">Plasmid Size</span></td><td>5100 bp</td></tr><tr><td><span class="info_label">Notes</span></td><td>Multiple cloning site for inserting gene of interest; Expression in mammalian cells and tissue</td></tr></tbody></table></td><td>5008<br>5009</td><td><span class="price" id="price_56483">$600.00</span>- 5 mg<br><span class="price" id="price_56374">$1,100.00</span>- 10 mg</td><td class="ordercell"><form action="https://606687.secure.netsuite.com/app/site/query/additemtocart.nl?c=606687&n=1&ext=T&continue= https://main--aldevron--hlxsites.hlx.page/catalog-products/reporters/traditional-pdna-reporters" method="post" data-hs-cf-bound="true">Quantity: <input id="qty" class="qtybox" name="qty" type="text" value="1"><br>Size:<select id="buyid" name="buyid"><option value="56483">5 mg</option><option value="56374">10 mg</option></select><br><button type="submit" class="addtocart" onclick='trackCart("5008/5009 gWiz-blank",this.parentNode.elements.buyid,this.parentNode.elements.qty.value)'>Add to Cart</button></form></td></tr></tbody></table>