Reporter Plasmids
In Stock Reporter Plasmids
Aldevron, your trusted partner since 1998, has several reporter plasmids available.
pALD-CV77-Luciferase Reporter
Consistent with the pALD-CV77 cloning backbone, Aldevron offers a pre-made reporter construct, pALD-CV77-Luciferase, which has several advantages:
- Commercially available sequence for research through clinical use
- Kanamycin resistance
- Consistent, reliable supply
- Immediately available at research grade
- Available in 100ug free sample vials, 5mg vials and 10mg vials, research grade only
gWiz Reporter Plasmids
The gWiz plasmids are designed for a wide range of mammalian cells and tissues. They contain a modified promoter, followed by intron A from the CMV immediate early gene, and an artificial transcription terminator. These ready-to-use plasmids can be applied directly to most in vitro and in vivo applications.
Each gWiz plasmid is ready to ship stocked at 5mg/mL in water enabling you to easily adjust to the buffer of your choice. Common features of the gWiz plasmids include:
- In vitro or in vivo gene expression
- CMV IE promoter
- Intron A
- Artificial transcription terminator
- Kanamycin resistance
- Available in 5mg and 10mg vials, research grade only
Quality & Ordering Information
pALD-CV77 Luciferase and gWiz Quality Control Specifications
| pALD-CV77-Luciferase | gWiz Reporter Plasmids | |
| Appearance | Clear and Colorless | Clear and colorless |
| Buffer | TE | Water |
| Bioburden | No growth in 48 hours at 37 +/- 2 C | N/A |
| Concentration | 1.0mg/mL +/- 10% | 5.0 mg/mL +/- 10% |
| DNA Homogeneity | ≥80% Supercoiled | Predominantly supercoiled |
| Endotoxin | ≤100 EU/mg | ≤ 100 EU/mg |
| Identity | Confirmed Against DNA Ladder | Confirmed Against DNA Ladder |
| Plasmid Identity | Identical to Reference Sequence | N/A |
| ABS 260/280 Ratio Purity | 1.80 – 2.00 | 1.80 - 2.00 |
| Residual Host Genomic DNA via qPCR | ≤5% | ≤ 5% |
| Residual Host Protein via BCA | ≤2% | Not visible at 200 ng load |
| Residual Host RNA via SYBR Gold | ≤5% by Semi-Quantitative Densitometry | N/A |
| Restriction Digest | Matches Expected Restriction Pattern | N/A |
| Mycoplasma Contamination | Negative for the Presence of Mycoplasma | N/A |
| Residual Host RNA | N/A | Not visible at 200 ng load |
Ordering Information
| Product | Pack Size | Catalog Number | RG Purchase Option |
| pALD-CV77-Luciferase - RG | 5 mg | 5078-5 | Buy Now |
| pALD-CV77-Luciferase - RG | 10 mg | 5078-10 | Buy Now |
| gWiz GFP - RG | 5 mg | 5006 | Buy Now |
| gWiz GFP - RG | 10 mg | 5007 | Buy Now |
| gWiz Luc- RG | 5 mg | 5000 | Buy Now |
| gWiz Luc - RG | 10 mg | 5001 | Buy Now |
| gWiz Blank - RG | 5 mg | 5008 | Buy Now |
| gWiz Blank - RG | 10 mg | 5009 | Buy Now |
