Enzyme Production

Custom Enzymes for Gene Editing

CRISPR-associated Nucleases

Aldevron offers custom manufacturing of CRISPR/Cas9 nucleases, including SpCas9 variants, SaCas9, dCas9 fusions and nickases. Custom variations of an existing Aldevron CRISPR nuclease are also available, including custom concentration, formulation or QC testing. Our manufacturing scale ranges from 10 mg to multi-gram lots.

Custom nucleases are supported by robust, scalable manufacturing protocols, including the capability of internally transferring from research grade to a cGMP facility. We also offer in-stock Research Grade, GMP-SourceTM and GMP CRISPR-associated nucleases in standard formulations for immediate delivery. These include SpyFiTM Cas9 Nuclease, SpCas9 and AsCpf1.

Enzymes for RNA Production

Our in vitro transcription (IVT) enzymes are qualified both by activity assays and functionally for RNA synthesis and can be custom manufactured to meet your specific project requirements from milligram to multigram scale. View the table below for a summary of our IVT enzymes.

Product Name Product Description

T7 RNA Polymerase

T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter.

Inorganic Pyrophosphatase

Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate.

Ribonuclease Inhibitor

Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C.

DNase I

DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.

Guanylyltransferase 

Catalyzes addition of 7-methylguanylate cap structures (Cap 0) to the 5’ end of RNA in three steps:

Reactions 1 & 2: Conversion of an RNA substrate pppN(pN)n to GpppN(pN)n with increasing amounts of enzyme was visualized by gel electrophoresis (uncapped, lower band; capped, upper band). One unit (U) is the amount of enzyme activity that caps 50% of a 5’-ppp oligo in 30 minutes at 37°C.

Reaction 3: Methyl transfer from SAM to GpppN(pN)n producing SAH and cap-0 mRNA was measured using the EPIgeneousTM Methyltransferase Assay (Cisbio). One unit (U) is the amount of enzyme activity that produces 1 pmol of SAH from SAM in 30 minutes at 37°C using cap-0 substrate.

2’-O-Methyltransferase

2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes
S-adenosylmethionine as the methyl group donor.

Poly(A) Polymerase

Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner.