Enzyme Production

Enzymes for Gene Editing

Cas9 nucleases from Aldevron provide high-quality protein for your CRISPR-Cas9 applications. The benefits of using Cas9 nucleases in protein form include reduced off-target effects, immediate activity (no need to transcribe and/or translate), and controlled dosage.

By incorporating nuclear localization signals on one or both termini, Cas9 nucleases traverse the nuclear membrane to edit DNA with high efficiency. Our Cas9 nucleases are designed for multiple delivery methods, including electroporation, transfection, microinjection and whole animal applications. In addition to the existing formats, Cas9 nucleases can be customized to suit specific needs.

Our Cas9 nuclease is a wild type S. pyogenes Cas9 protein. Cas9 protein and guide RNA form a ribonucleoprotein (RNP) complex that identifies a specific site and cleaves double-stranded DNA. Since transcription and translation are not required, the Cas9 complex can quickly perform its function and rapidly clear from the cell.

The following Cas9 products are available in pre-defined formulations and custom options.

Enzymes for RNA Production

Our in vitro transcription (IVT) enzymes are qualified both by activity assays and functionally for RNA synthesis and can be custom manufactured to meet your specific project requirements from milligram to multigram scale. View the table below for a summary of our IVT enzymes.

Product Name Product Description

T7 RNA Polymerase

T7 RNA Polymerase catalyzes synthesis of RNA in the presence of a DNA template containing the T7 phage promoter.

Inorganic Pyrophosphatase

Inorganic Pyrophosphatase catalyzes hydrolysis of inorganic pyrophosphate, producing two molecules of phosphate.

Ribonuclease Inhibitor

Ribonuclease Inhibitor is a potent, noncompetitive inhibitor of pancreatic-type ribonucleases including ribonucleases A, B and C.

DNase I

DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.

Capping Enzyme

Vaccinia Capping Enzyme (Guanylyltransferase) adds the 7-methylguanylate cap to the 5' end of RNA. The heterodimeric enzyme has three distinct enzymatic activities: RNA triphosphatase (reaction 1), guanylyltransferase (reaction 2) and guanine methyltransferase (reaction 3). Each activity is required for formation of the m7Gppp5'N cap.

2’-O-Methyltransferase

2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes
S-adenosylmethionine as the methyl group donor.

Poly(A) Polymerase

Poly(A) Polymerase catalyzes addition of AMP from ATP to the 3' end of RNA in a template-independent manner.