Plasmid DNA Production

Plasmid DNA for research, preclinical, and clinical applications scaled to fit projects of any size

Aldevron has been perfecting plasmid DNA production for nearly 20 years, successfully completing nearly 70,000 projects to date. Using proprietary technology, we manufacture DNA for a wide range of research, preclinical, clinical, and diagnostic applications.

Whether you require 1mg of DNA for a research study or 100 g for a clinical trial, our goal is to be your vendor of choice and provide you with the most affordable, high-quality plasmid DNA manufacturing services that meet your requirements.

Aldevron's Plasmid DNA Process

Research-Grade Plasmid DNA

We manufacture high-quality plasmid DNA, linearized vectors, minicircle DNA, and thousands of preparations per day for high-throughput screening.

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GMP-Source™ Plasmid DNA

Discover how GMP-Source™ plasmid DNA retains the key components of cGMP manufacturing to offer a faster and cost-effective alternative to GMP plasmids.

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GMP Plasmid DNA

Our customized GMP plasmid DNA manufacturing is driven by a comprehensive quality assurance process to ensure the highest quality products with each and every order.

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Applications

Transfection

Pure, high-quality plasmid DNA is critical for a successful transfection. Our high-purity, high-homogeneity, low-endotoxin plasmid DNA is ideal for any transfection application.

Case Study: A client suffered from very low plasmid production yields using other vendors. Our optimization resulted in a 9X increase in the yield per liter of media. In addition, the purity far exceeded that of previous manufacturing attempts.

Gene therapy

From ITR-bearing cis-plasmids to very large helper plasmids, our manufacturing technology produces high yields even for the most difficult constructs.

Case Study: A client required 95% monomer plasmid DNA as the final deliverable. The starting material provided was predominantly dimerized/multimeric. Our team linearized the material, ligated it back together to create the monomer, and transformed. We achieved the required specification for both the supercoiled and linearized plasmid DNA deliverable.

In vivo studies for DNA vaccines

With a consistent manufacturing process from research grade through clinical production, we ensure reliable, high-quality DNA for your vaccine program.

Case Study: When West Nile virus threatened the survival of endangered condors in California, we were contracted to manufacture a DNA vaccine developed by the Centers for Disease Control and Prevention. Our production and quality control systems allowed us to manufacture the vaccine in less than two months. The vaccine went on to protect other endangered species against West Nile virus.

RNA template

Whether your application is a messenger RNA therapeutic or guide RNA for CRISPR-Cas9, we have extensive experience producing linearized plasmid DNA using a variety of enzymes with advanced purification techniques.

Case Study: A client required a high yield and consistent poly(A) tail length for their template. We evaluated a variety of resin and buffer combinations as well as post-linearization purification strategies. The result was 90-95% recovery of linearized DNA with a consistently high poly(A) tail length.

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