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Protein Production Capabilities
Aldevron offers a wide range of protein services including host cell optimization, expression screening (cells, media, construct, culturing conditions), process development, contract production, enzyme and antibody assay design, and more. Proteins produced by Aldevron are used for a wide range of agriculture and biomedical applications.
Whether we use your process or develop a new one to meet your needs, our goal is to provide the highest-quality protein while meeting your time and budget requirements. Aldevron's protein production capabilities extend from simple research through to full cGMP. With over 80 years of combined expertise, our protein team will provide the solution that is right for you.
| Project | Application | Aldevron's Advantage |
|---|---|---|
| >300 g of protein for USDA- sponsored feed study | Safety study for new transgenic plant | Complete method development, scale and lyophylization capability accelerated time to market |
| Superantigen Staph. enterotoxins | Vaccine studies, upcoming clinical trial support | CDC registered production facilities and ABSL2 laboratory enabled onsite manufacturing and testing |
| MMP enzymes | MMP family used widely in biomedical research | Unique method development required to minimize instability during production across broad scale manufacture |
| Parallel construct screening | Screened multiple constructs for optimal protein yield and activity | Optimized the relationship between host, construct, culturing conditions and media |
| 10 L protein expression scale-up in insect cells | Structural studies | Proteins produced in WAVE™ BioReactors Included 10 L scale-up expression of target clone |
Contract Protein Production
- Primer design and preparation
- Gene synthesis and vector preparation
- Mutagenesis
- Custom vectors
MCB/MWCB
- Generation
- Characterization
- Retention
- Stability
- Yield optimization
- Folding
- Purity
Multiple host systems
- E. coli, Insect, Mammalian
- Scale — 1ml to 240L
- OEM production, filling and labeling
- Assay development
- High-titer stock generation
- Chemical/physical lysis
- Dialysis/centrifugation/TFF
- Chromatography — ion exchange, hydrophobic interaction, hydroxyapatite, size exclusion
- Lyophilization/fill and finish
- Ultrafiltration/diafiltration
Quality Testing
Aldevron offers a broad range of assays to test the quality of your protein in process and final release. In addition, client specified categories including formulation, stability, and toxicology testing are available upon request.
| Category | Assay Method |
|---|---|
| Concentration: | A280, BCA, Bradford assay |
| Purity | SDS-PAGE, Coomassie® staining, silver staining, western blotting, HPLC, and mass spectrometry analysis |
| Endotoxin level | Limulus amebocyte assay (LAL) |
| Sterility | Bioburden, USP testing |
| Activity | As requested |
Scale Up and Method Development
Aldevron's protein team is experienced with ramping up existing processes and developing new and unique purification strategies. For “scale-up” projects, we identify process engineering limitations and rapidly develop the most cost effective, robust solutions based on your existing platform and product release specifications.