Ultra-Pure - Ready to Use
Genlantis Inc., has engineered a new series of reporter plasmids based on the gWiz™ high-expression vector system. These plasmids have been designed to produce the highest levels of gene expression in a wide range of mammalian cells and tissues. They contain a proprietarily modified promoter followed by intron A from the CMV immediate early gene and a high-efficiency artificial transcription terminator. The expression vectors are constructed in a plasmid backbone which has been extensively modified to achieve enhanced levels of transgene expression in mammalian cells.

Milligram Amounts Available
Aldevron now offers ultra-pure, milligram amounts of these plasmids for use by Gene Therapy and DNA Vaccine researchers. Aldevron's exclusive fermentation technology and DNA purification process are used to produce the gWiz™ High-Expression Reporter Plasmids. The ready-to-use plasmids are endotoxin-free and can be applied directly to most in vitro and in vivo applications. Plasmids can be delivered in the buffer of your choice for an additional charge of $25. Material otherwise will ship in water. The turn-around time is approximately 24 to 48 hours. Significant volume discounts are available. Please inquire for further details.

Cell types as indicated were transfected in 24-well plates with 1 ug of the gWiz B-galactosidase vector or 1 ug of the CMV-1 or CMV-2 vectors (commercial vectors with CMV IE promoter regulating the B-gal gene) and 5 ul of GenePORTER transfection reagent. For Jurkat cells, 8 ul of GenePORTER reagent was used. The graph for each transfection represents the mean of two separate experiments in which each sample was tested in duplicate. B-galactosidase expression was measured 48 hours after transfection by colorimetric assay. For comparison purposes, in each cell type, the levels of B-gal expression were adjusted to show relative expression by normalizing the B-gal expression of the gWiz vector to 100%.