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plasmids express the Hepatitis B Surface Antigen (HBsAg) under the
control of the CMV promoter and are designed to validate DNA vaccine
methods. DNA Vaccine Plasmids are available free-of-charge.
pRc/CMV-HBs(S)
One of the most widely used plasmids in DNA
vaccine research.
One of the most widely used plasmids in DNA vaccine
research, pRc/CMV-HBs(S) (or simply pCMV-S)
expresses the hepatitis B surface antigen (small, or S, protein) under
the control of the CMV immediate-early promoter. This plasmid is freely
available upon request for validating DNA-based immunization methods
and other experimental uses. Antibodies generated to the hepatitis
B surface antigen can be measured using ELISA and the results can subsequently
be compared to published data.
pCMV-S has been widely used in Gene Therapy and DNA vaccine studies.
These include:
- novel DNA vaccine delivery route studies.
- liposome, formulation,
and stability studies.
- adjuvant studies.
Please contact us for a current list of pCMV-S references.
These will enable you to compare your
data to published results.
Reference:
Please see the following pCMV-S reference
for details of its construction and use:
Davis HL, Michel ML, Whalen RG. DNA-based immunization for Hepatitis
B induces continuous secretion of antigen and high levels of circulating
antibody. Human Molecular Genetics. 1993. 2: 1847-1851.
Also, a helpful reprint can be downloaded by clicking
here.
If you have trouble downloading this
document, please contact us and
we will be happy to email you a copy.
Please cite this reference when appropriate.
Ready to use DNA
Aldevron can provide you with milligram
amounts of ultrapure pCMV-S in the
buffer of your choice through our
custom production service. We offer rapid delivery times and significant
volume discounts for this and any other plasmid. Once the plasmid
is shipped to you, it will be ready for immediate use. You will not
have to spend your valuable time making DNA. To place an order or
to obtain price information, please contact us.
Free DNA
Aldevron can provide you with 2-4 micrograms
of this plasmid free of charge. For
instructions, please see the plasmid
DNA request form. A nominal
shipping fee may apply for international orders.
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