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Antibody Processing
Aldevron offers the production and purification of antibodies with the highest quality. A wide range of antibody processing and characterization methods are also available. Each service can be included as part of an antibody development project, or provided as a standalone service.- Production & Purification
Antibody Production & Purification
- Production from client hybridomas grown in serum-free media
- Expression in mammalian cell lines transfected with heavy & light chains
- Scale-up production from hybridoma supernatant and antisera (peptide, protein IgG)
- Mycoplasma testing
- Endotoxin testing and removal
- SDS-PAGE
- Western blot
- Antibody Processing
Antibody Processing
- Fragmentation, Fab & F(ab')2
- DNA sequencing of heavy & light chains
- Labeling (biotin, fluorophore, peroxidase)
- Humanization
- Antibody Characterization
Antibody Characterization
- Isotyping
- Cross-reactivity (inter- & intra species)
- KD50
- Epitope mapping
- Identification of ELISA sandwich pairs
- Confocal laser microscopy
- Immunohistochemistry